Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme - PubMed (original) (raw)

Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme

Mark A Toleman et al. J Antimicrob Chemother. 2002 Nov.

Abstract

The gene encoding the metallo-beta-lactamase SPM-1 was cloned from a genomic library of Pseudomonas aeruginosa strain 48-1997 A. The insert carrying spm-1 possessed a GC content of 47%, indicating that it is of non-Pseudomonas origin. Upstream of spm-1 there is a small open reading frame (ORF), which is homologous to the LysR family of proteins (69% identity to the LysR protein from Salmonella enterica serovar Typhimurium). Downstream of spm-1 there is the start of an ORF, the product of which shows close homology with the GroEL-type proteins from Xanthomonas campestris. No transmissible element could be identified upstream or downstream of spm-1. The spm-1 gene is carried on a plasmid that can transform both Escherichia coli and P. aeruginosa to ceftazidime resistance. SPM-1 contains the classic metallo-beta-lactamase zinc-binding motif HXHXD and shows the highest identity (35.5%) to IMP-1. SPM-1 is a distinctly different metallo-beta-lactamase from VIM and IMP and, accordingly, represents a new subfamily of mobile metallo-beta-lactamases. The predicted molecular weight of the protein was 27 515 Da, significantly higher than that of IMP (25 041 Da) or VIM (25 322 Da). SPM-1 possesses a unique loop of 23 residues that accounts for the higher molecular mass.

PubMed Disclaimer

Cited by

Antibiotic Resistance Profiles, Molecular Mechanisms and Innovative Treatment Strategies of Acinetobacter baumannii.
Vrancianu CO, Gheorghe I, Czobor IB, Chifiriuc MC. Vrancianu CO, et al. Microorganisms. 2020 Jun 21;8(6):935. doi: 10.3390/microorganisms8060935. Microorganisms. 2020. PMID: 32575913 Free PMC article. Review.
Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-beta-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp.
Lee K, Lim YS, Yong D, Yum JH, Chong Y. Lee K, et al. J Clin Microbiol. 2003 Oct;41(10):4623-9. doi: 10.1128/JCM.41.10.4623-4629.2003. J Clin Microbiol. 2003. PMID: 14532193 Free PMC article.
Biochemical characterization of the acquired metallo-beta-lactamase SPM-1 from Pseudomonas aeruginosa.
Murphy TA, Simm AM, Toleman MA, Jones RN, Walsh TR. Murphy TA, et al. Antimicrob Agents Chemother. 2003 Feb;47(2):582-7. doi: 10.1128/AAC.47.2.582-587.2003. Antimicrob Agents Chemother. 2003. PMID: 12543663 Free PMC article.
CRISPR-Based Gene Editing in Acinetobacter baumannii to Combat Antimicrobial Resistance.
Junaid M, Thirapanmethee K, Khuntayaporn P, Chomnawang MT. Junaid M, et al. Pharmaceuticals (Basel). 2023 Jun 23;16(7):920. doi: 10.3390/ph16070920. Pharmaceuticals (Basel). 2023. PMID: 37513832 Free PMC article. Review.
A 7-Year Brazilian National Perspective on Plasmid-Mediated Carbapenem Resistance in Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii Complex and the Impact of the Coronavirus Disease 2019 Pandemic on Their Occurrence.
Kiffer CRV, Rezende TFT, Costa-Nobre DT, Marinonio ASS, Shiguenaga LH, Kulek DNO, Arend LNVS, Santos ICO, Sued-Karam BR, Rocha-de-Souza CM, Kraft L, Abreu A, Peral RTS, Carvalho-Assef APD, Pillonetto M. Kiffer CRV, et al. Clin Infect Dis. 2023 Jul 5;77(Suppl 1):S29-S37. doi: 10.1093/cid/ciad260. Clin Infect Dis. 2023. PMID: 37406041 Free PMC article.

Molecular characterization of SPM-1, a novel metallo-beta-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme - PubMed (original) (raw)