Immunosuppression with cyclosporin A alters the thymic microenvironment - PubMed (original) (raw)
Immunosuppression with cyclosporin A alters the thymic microenvironment
M Kanariou et al. Clin Exp Immunol. 1989 Nov.
Abstract
The effect of cyclosporin A (CyA) immunosuppression on the murine thymic microenvironment and T lymphocyte development has been analysed using monoclonal antibodies to epithelial and lymphocyte subpopulations, macrophages and major histocompatibility complex (MHC) class II antigens in immunohistochemistry and flow cytometry. The major microenvironmental target for CyA-induced damage was the thymic medulla, where a reduction in all epithelial cell subsets, dendritic cells and macrophages was observed. In contrast, the thymic cortex appeared essentially normal. CyA had no detectable effect on the intensity of microenvironmental expression of MHC class II molecules in either cortex or medulla, although the number of MHC class II positive medullary cells was reduced after CyA treatment. CyA also had a differential effect on the thymic lymphocyte populations where there was little change in the Thy-1 bright, CD5 dull, CD4+, CD8+ cortical thymocytes but a depletion of the Thy-1 dull, CD5 bright, CD4 or CD8 single-positive medullary cells. This lymphocyte loss may be due partly to increased migration from thymus to spleen and other peripheral lymphoid organs, and partly to a block in the differentiation stage from cortical to medullary lymphocyte. The thymic microenvironment and lymphocyte subpopulations recover rapidly after cessation of CyA treatment, although there may be longer term functional defects resulting from the CyA-induced injury.
Similar articles
- Immunohistology of lymphoid and non-lymphoid cells in the thymus in relation to T lymphocyte differentiation.
Van Ewijk W. Van Ewijk W. Am J Anat. 1984 Jul;170(3):311-30. doi: 10.1002/aja.1001700307. Am J Anat. 1984. PMID: 6332520 - The role of the reticulo-epithelial (RE) cell network in the immuno-neuroendocrine regulation of intrathymic lymphopoiesis.
Bodey B, Bodey B Jr, Siegel SE, Kaiser HE. Bodey B, et al. Anticancer Res. 2000 May-Jun;20(3A):1871-88. Anticancer Res. 2000. PMID: 10928121 - Effects of cyclosporin A on mouse thymus: immunochemical and ultrastructural studies.
Fabien NH, Auger C, Moreira A, Monier JC. Fabien NH, et al. Thymus. 1992 Nov;20(3):153-62. Thymus. 1992. PMID: 1462361 - Cyclosporin A-induced changes of the thymic microenvironment. A review of morphological studies.
Milićević NM, Milićević Z. Milićević NM, et al. Histol Histopathol. 1998 Oct;13(4):1183-96. doi: 10.14670/HH-13.1183. Histol Histopathol. 1998. PMID: 9810510 Review. - Thymic microenvironments for T-cell repertoire formation.
Nitta T, Murata S, Ueno T, Tanaka K, Takahama Y. Nitta T, et al. Adv Immunol. 2008;99:59-94. doi: 10.1016/S0065-2776(08)00603-2. Adv Immunol. 2008. PMID: 19117532 Review.
Cited by
- Failures in thymus medulla regeneration during immune recovery cause tolerance loss and prime recipients for auto-GVHD.
Alawam AS, Cosway EJ, James KD, Lucas B, Bacon A, Parnell SM, White AJ, Jenkinson WE, Anderson G. Alawam AS, et al. J Exp Med. 2022 Feb 7;219(2):e20211239. doi: 10.1084/jem.20211239. Epub 2021 Dec 15. J Exp Med. 2022. PMID: 34910105 Free PMC article. - Selection of antibodies to cell surface determinants on mouse thymic epithelial cells using a phage display library.
Palmer DB, George AJ, Ritter MA. Palmer DB, et al. Immunology. 1997 Jul;91(3):473-8. doi: 10.1046/j.1365-2567.1997.00262.x. Immunology. 1997. PMID: 9301539 Free PMC article. - The cyclosporins.
Rehácek Z. Rehácek Z. Folia Microbiol (Praha). 1995;40(1):68-88. doi: 10.1007/BF02816529. Folia Microbiol (Praha). 1995. PMID: 8851466 Review. - The effects of pregnancy on the mouse thymic epithelium.
Clarke AG, Gil AL, Kendall MD. Clarke AG, et al. Cell Tissue Res. 1994 Feb;275(2):309-18. doi: 10.1007/BF00319429. Cell Tissue Res. 1994. PMID: 8111839 - Developmental changes predispose the fetal thymus to positive selection of CD4+CD8- T cells.
Fairchild PJ, Austyn JM. Fairchild PJ, et al. Immunology. 1995 Jun;85(2):292-8. Immunology. 1995. PMID: 7642219 Free PMC article.
References
- J Exp Med. 1986 Nov 1;164(5):1615-25 - PubMed
- J Exp Med. 1988 Nov 1;168(5):1741-7 - PubMed
- J Immunol. 1985 Aug;135(2):1165-71 - PubMed
- Immunology. 1986 Dec;59(4):621-5 - PubMed
- Am J Pathol. 1987 Mar;126(3):487-96 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous