Phagocytosis and killing of bacteria by professional phagocytes and dendritic cells - PubMed (original) (raw)

Phagocytosis and killing of bacteria by professional phagocytes and dendritic cells

Markus Nagl et al. Clin Diagn Lab Immunol. 2002 Nov.

Abstract

Dendritic cells (DC) represent a class of professional antigen-presenting cells whose primary function is to alert the immune system, not to clear invading microorganisms. The objective of our study was to compare the abilities of polymorphonuclear neutrophilic leukocytes (PMN), monocytes, monocyte-derived macrophages (MDM), monocyte-derived immature DC (imDC), and mature DC (maDC) to ingest and destroy Staphylococcus aureus and Escherichia coli. Acridine orange staining and fluorescence microscopy demonstrated that MDM, followed by monocytes, imDC, and PMN, internalized bacteria well but that maDC exhibited less pronounced phagocytic activity. PMN, monocytes, and MDM exhibited a much higher capacity to kill ingested bacteria than both imDC and maDC. In summary, these data are in agreement with the generally accepted idea that different types of leukocytes fulfill specialized tasks in antigen presentation and killing of pathogens.

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Figures

FIG. 1.

FIG. 1.

Intracellular (A and B) and extracellular (C and D) counts of viable bacteria after phagocytosis. Opsonized bacteria (1.9 × 105 to 1.1 × 106 CFU/ml for S. aureus and 8.9 × 104 to 4.0 × 105 CFU/ml for E. coli) were incubated with cells (1 × 106/ml) at 37°C under continuous rotation. Subsequent to centrifugation for 4 min at 110 × g, quantitative cultures of the supernatant were performed to evaluate the extracellular number of bacteria. Intracellular counts were done after two washing steps and lysis of the phagocytes in the pellet with 0.5% NP-40 for 10 min. Results are the means ± standard errors of the means of results from three independent experiments. Symbols: ▪, PMN; ▴, monocytes; ▾, MDM; ◊, imDC; ○, maDC; *, P < 0.05 compared to results for PMN; **, P < 0.01 compared to results for PMN.

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