Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases - PubMed (original) (raw)

Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases

Laurent Poirel et al. Antimicrob Agents Chemother. 2002 Dec.

Abstract

A chromosome-encoded beta-lactamase gene, cloned and expressed in Escherichia coli from Kluyvera georgiana reference strain CUETM 4246-74 (DSM 9408), encoded the extended-spectrum beta-lactamase KLUG-1, which shared 99% amino acid identity with the plasmid-mediated beta-lactamase CTX-M-8. This work provides further evidence that Kluyvera spp. may be the progenitor(s) of CTX-M-type beta-lactamases.

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Figures

FIG. 1.

Alignment of the KLUG-1 amino acid sequence with that of CTX-M-8 from C. amalonaticus (3). The numbering is according to the scheme of Ambler et al. (1). Dashes represent identical amino acid residues. The vertical arrow is the putative cleavage site of the leader peptide of the mature β-lactamase KLUG-1. Three structural elements characteristic of class A β-lactamases are boxed in gray (9).

FIG. 2.

Comparison of the surrounding DNA sequences of _bla_KLUG-1 and bla_CTX-M-8 of recombinant plasmid pKG-2 and natural plasmid pRio-2, respectively. Boldfaced nucleotides indicate start and stop codons of β-lactamase genes. Vertical bars indicate identical nucleotides, whereas dots represent deleted nucleotides. IS_10 IRR refers to the inverted repeat right of an IS_10_-like element.

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References

1. Ambler, R. P., A. F. W. Coulson, J.-M. Frère, J. M. Ghuysen, B. Joris, M. Forsman, R. C. Lévesque, J. Tiraby, and S. G. Waley. 1991. A standard numbering scheme for the class A β-lactamases. Biochem. J. 276:269-270. - PMC - PubMed
1. Bonnet, R., C. Dutour, J. L. M. Sampaio, C. Chanal, D. Sirot, R. Labia, C. De Champs, and J. Sirot. 2000. Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240 to Gly. Antimicrob. Agents Chemother. 45:2269-2275. - PMC - PubMed
1. Bonnet, R., J. L. M. Sampaio, R. Labia, C. De Champs, D. Sirot, C. Chanal, and J. Sirot. 2000. A novel CTX-M β-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil. Antimicrob. Agents Chemother. 44:1936-1942. - PMC - PubMed
1. Chanawong, A., F. H. M'Zali, J. Heritage, J.-X. Xiong, and P. M. Hawkey. 2002. Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China. Antimicrob. Agents Chemother. 46:630-637. - PMC - PubMed
1. Decousser, J.-W., L. Poirel, and P. Nordmann. 2001. Characterization of a chromosomally encoded extended-spectrum class A β-lactamase from Kluyvera cryocrescens. Antimicrob. Agents Chemother. 45:3595-3598. - PMC - PubMed

Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases - PubMed (original) (raw)