Long-term multiple color imaging of live cells using quantum dot bioconjugates - PubMed (original) (raw)
doi: 10.1038/nbt767. Epub 2002 Dec 2.
Affiliations
- PMID: 12459736
- DOI: 10.1038/nbt767
Long-term multiple color imaging of live cells using quantum dot bioconjugates
Jyoti K Jaiswal et al. Nat Biotechnol. 2003 Jan.
Abstract
Luminescent quantum dots (QDs)--semiconductor nanocrystals--are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells. The two approaches presented are (i) endocytic uptake of QDs and (ii) selective labeling of cell surface proteins with QDs conjugated to antibodies. Live cells labeled using these approaches were used for long-term multicolor imaging. The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development.
Comment in
- Quantum dots finally come of age.
Jovin TM. Jovin TM. Nat Biotechnol. 2003 Jan;21(1):32-3. doi: 10.1038/nbt0103-32. Nat Biotechnol. 2003. PMID: 12511905 No abstract available.
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