Complementation of the nuclear antisense rbcS-induced photosynthesis deficiency by introducing an rbcS gene into the tobacco plastid genome - PubMed (original) (raw)
. 2002 Nov;43(11):1302-13.
doi: 10.1093/pcp/pcf158.
Affiliations
- PMID: 12461130
- DOI: 10.1093/pcp/pcf158
Complementation of the nuclear antisense rbcS-induced photosynthesis deficiency by introducing an rbcS gene into the tobacco plastid genome
Xing-Hai Zhang et al. Plant Cell Physiol. 2002 Nov.
Abstract
The small subunit (SS) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a nuclear gene-encoded protein that is imported into chloroplasts where it assembles with the large subunit (LS) after removal of the transit peptide to form Rubisco. We have explored the possibility that the severe deficiency in photosynthesis exhibited in nuclear transgenic tobacco (line alpha5) expressing antisense rbcS coding DNA that results in low SS and Rubisco protein content [Rodermel et al. (1988) Cell 55: 673] could be complemented by introducing a copy of the rbcS gene into its plastid genome through chloroplast transformation. Two independent lines of transplastomic plants were generated, in which the tobacco rbcS coding sequence, either with or without the transit sequence, was site-specifically integrated into the plastid genome. We found that compared with the antisense plants, expression of the plastid rbcS gene in the transplastomic plants resulted in very high mRNA abundance but no increased accumulation of the SS and Rubisco protein or improvement in plant growth and photosynthesis. Therefore, there is a limitation in efficient translation of the rbcS mRNA in the plastid or an incorrect processing and modification of the plastid-synthesized SS protein that might cause its rapid degradation.
Similar articles
- Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit translation is regulated in a small subunit-independent manner in the expanded leaves of tobacco.
Ichikawa K, Miyake C, Iwano M, Sekine M, Shinmyo A, Kato K. Ichikawa K, et al. Plant Cell Physiol. 2008 Feb;49(2):214-25. doi: 10.1093/pcp/pcm179. Epub 2008 Jan 4. Plant Cell Physiol. 2008. PMID: 18178584 - Hybrid Rubisco of tomato large subunits and tobacco small subunits is functional in tobacco plants.
Zhang XH, Webb J, Huang YH, Lin L, Tang RS, Liu A. Zhang XH, et al. Plant Sci. 2011 Mar;180(3):480-8. doi: 10.1016/j.plantsci.2010.11.001. Epub 2010 Nov 13. Plant Sci. 2011. PMID: 21421395 - A mechanism for intergenomic integration: abundance of ribulose bisphosphate carboxylase small-subunit protein influences the translation of the large-subunit mRNA.
Rodermel S, Haley J, Jiang CZ, Tsai CH, Bogorad L. Rodermel S, et al. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3881-5. doi: 10.1073/pnas.93.9.3881. Proc Natl Acad Sci U S A. 1996. PMID: 8632983 Free PMC article. - Chloroplast transformation for engineering of photosynthesis.
Hanson MR, Gray BN, Ahner BA. Hanson MR, et al. J Exp Bot. 2013 Jan;64(3):731-42. doi: 10.1093/jxb/ers325. Epub 2012 Nov 16. J Exp Bot. 2013. PMID: 23162121 Review. - Manipulating ribulose bisphosphate carboxylase/oxygenase in the chloroplasts of higher plants.
John Andrews T, Whitney SM. John Andrews T, et al. Arch Biochem Biophys. 2003 Jun 15;414(2):159-69. doi: 10.1016/s0003-9861(03)00100-0. Arch Biochem Biophys. 2003. PMID: 12781767 Review.
Cited by
- An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update.
Mahapatra K, Banerjee S, De S, Mitra M, Roy P, Roy S. Mahapatra K, et al. Front Cell Dev Biol. 2021 Aug 10;9:671698. doi: 10.3389/fcell.2021.671698. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34447743 Free PMC article. Review. - Advancing organelle genome transformation and editing for crop improvement.
Li S, Chang L, Zhang J. Li S, et al. Plant Commun. 2021 Jan 4;2(2):100141. doi: 10.1016/j.xplc.2021.100141. eCollection 2021 Mar 8. Plant Commun. 2021. PMID: 33898977 Free PMC article. Review. - Modifying Plant Photosynthesis and Growth via Simultaneous Chloroplast Transformation of Rubisco Large and Small Subunits.
Martin-Avila E, Lim YL, Birch R, Dirk LMA, Buck S, Rhodes T, Sharwood RE, Kapralov MV, Whitney SM. Martin-Avila E, et al. Plant Cell. 2020 Sep;32(9):2898-2916. doi: 10.1105/tpc.20.00288. Epub 2020 Jul 9. Plant Cell. 2020. PMID: 32647068 Free PMC article. - Production of functional native human interleukin-2 in tobacco chloroplasts.
Zhang XH, Keating P, Wang XW, Huang YH, Martin J, Hartmann JX, Liu A. Zhang XH, et al. Mol Biotechnol. 2014 Apr;56(4):369-76. doi: 10.1007/s12033-013-9717-x. Mol Biotechnol. 2014. PMID: 24146433 - Plastid genetic engineering in Solanaceae.
Venkatesh J, Park SW. Venkatesh J, et al. Protoplasma. 2012 Oct;249(4):981-99. doi: 10.1007/s00709-012-0391-9. Epub 2012 Mar 7. Protoplasma. 2012. PMID: 22395455 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources