Ceramide increases oxidative damage due to inhibition of catalase by caspase-3-dependent proteolysis in HL-60 cell apoptosis - PubMed (original) (raw)
. 2003 Mar 14;278(11):9813-22.
doi: 10.1074/jbc.M201867200. Epub 2003 Jan 2.
Affiliations
- PMID: 12511568
- DOI: 10.1074/jbc.M201867200
Free article
Ceramide increases oxidative damage due to inhibition of catalase by caspase-3-dependent proteolysis in HL-60 cell apoptosis
Kazuya Iwai et al. J Biol Chem. 2003.
Free article
Abstract
We investigated through which mechanisms ceramide increased oxidative damage to induce leukemia HL-60 cell apoptosis. When 5 microm N-acetylsphingosine (C(2)-ceramide) or 20 microm H(2)O(2) alone induced little increase of reactive oxygen species (ROS) generation as judged by the 2'-7'-dichlorofluorescin diacetate method, 20 microm H(2)O(2) enhanced oxidative damage as judged by ROS accumulation, and thiobarbituric acid-reactive substance production after pretreatment with 5 microm C(2)-ceramide at least for 12 h. The treatment with a catalase inhibitor, 3-amino-1h-1,2,4-triazole, increased oxidative damage and apoptosis induced by H(2)O(2), and in contrast, purified catalase inhibited the enhancement of oxidative damage by H(2)O(2) in ceramide-pretreated cells, suggesting that the oxidative effect of ceramide is involved in catalase regulation. Indeed, C(2)-ceramide inhibited the activity of immunoprecipitated catalase and decreased the levels of catalase protein in a time-dependent manner. Moreover, acetyl-Asp-Met-Gln-Asp-aldehyde, which dominantly inhibited caspase-3 and blocked the increase of oxidative damage and apoptosis due to C(2)-ceramide-induced catalase depletion at protein and activity levels. In vitro, active and purified caspase-3, but not caspase-6, -8, and -9, inhibited catalase activity and induced the proteolysis of catalase protein whereas these in vitro effects of caspase-3 were blocked by acetyl-Asp-Met-Gln-Asp-aldehyde. Taken together, it is suggested that H(2)O(2) enhances apoptosis in ceramide-pretreated cells, because ceramide increases oxidative damage by inhibition of ROS scavenging ability through caspase-3-dependent proteolysis of catalase.
Similar articles
- Vesnarinone causes oxidative damage by inhibiting catalase function through ceramide action in myeloid cell apoptosis.
Kondo T, Suzuki Y, Kitano T, Iwai K, Watanabe M, Umehara H, Daido N, Domae N, Tashima M, Uchiyama T, Okazaki T. Kondo T, et al. Mol Pharmacol. 2002 Mar;61(3):620-7. doi: 10.1124/mol.61.3.620. Mol Pharmacol. 2002. PMID: 11854443 - Control of ceramide-induced apoptosis by IGF-1: involvement of PI-3 kinase, caspase-3 and catalase.
Kondo T, Kitano T, Iwai K, Watanabe M, Taguchi Y, Yabu T, Umehara H, Domae N, Uchiyama T, Okazaki T. Kondo T, et al. Cell Death Differ. 2002 Jun;9(6):682-92. doi: 10.1038/sj.cdd.4401019. Cell Death Differ. 2002. PMID: 12032677 - Ceramide-induced apoptosis: role of catalase and hepatocyte growth factor.
Kannan R, Jin M, Gamulescu MA, Hinton DR. Kannan R, et al. Free Radic Biol Med. 2004 Jul 15;37(2):166-75. doi: 10.1016/j.freeradbiomed.2004.04.011. Free Radic Biol Med. 2004. PMID: 15203188 - Life and death decisions: ceramide generation and EGF receptor trafficking are modulated by oxidative stress.
Goldkorn T, Ravid T, Khan EM. Goldkorn T, et al. Antioxid Redox Signal. 2005 Jan-Feb;7(1-2):119-28. doi: 10.1089/ars.2005.7.119. Antioxid Redox Signal. 2005. PMID: 15650401 Review. - Ceramides as Mediators of Oxidative Stress and Inflammation in Cardiometabolic Disease.
Gaggini M, Ndreu R, Michelucci E, Rocchiccioli S, Vassalle C. Gaggini M, et al. Int J Mol Sci. 2022 Feb 28;23(5):2719. doi: 10.3390/ijms23052719. Int J Mol Sci. 2022. PMID: 35269861 Free PMC article. Review.
Cited by
- Multi-Effects of Acute Salinity Stress on Osmoregulation, Physiological Metabolism, Antioxidant Capacity, Immunity, and Apoptosis in Macrobrachium rosenbergii.
Wang Y, Li H, Wei J, Hong K, Zhou Q, Liu X, Hong X, Li W, Liu C, Zhu X, Yu L. Wang Y, et al. Antioxidants (Basel). 2023 Oct 7;12(10):1836. doi: 10.3390/antiox12101836. Antioxidants (Basel). 2023. PMID: 37891915 Free PMC article. - Ceramide induces MMP-9 expression through JAK2/STAT3 pathway in airway epithelium.
Xuan L, Han F, Gong L, Lv Y, Wan Z, Liu H, Ren L, Yang S, Zhang W, Li T, Tan C, Liu L. Xuan L, et al. Lipids Health Dis. 2020 Aug 24;19(1):196. doi: 10.1186/s12944-020-01373-w. Lipids Health Dis. 2020. PMID: 32829707 Free PMC article. - Yeast polyubiquitin gene UBI4 deficiency leads to early induction of apoptosis and shortened replicative lifespan.
Zhao W, Zhou T, Zheng HZ, Qiu KP, Cui HJ, Yu H, Liu XG. Zhao W, et al. Cell Stress Chaperones. 2018 Jul;23(4):527-537. doi: 10.1007/s12192-017-0860-3. Epub 2017 Nov 7. Cell Stress Chaperones. 2018. PMID: 29116578 Free PMC article. - β-Sitosterol and stigmasterol ameliorate dextran sulfate sodium-induced colitis in mice fed a high fat Western-style diet.
Feng S, Dai Z, Liu A, Wang H, Chen J, Luo Z, Yang CS. Feng S, et al. Food Funct. 2017 Nov 15;8(11):4179-4186. doi: 10.1039/c7fo00375g. Food Funct. 2017. PMID: 29034917 Free PMC article. - Long chain saturated and unsaturated fatty acids exert opposing effects on viability and function of GLP-1-producing cells: Mechanisms of lipotoxicity.
Thombare K, Ntika S, Wang X, Krizhanovskii C. Thombare K, et al. PLoS One. 2017 May 16;12(5):e0177605. doi: 10.1371/journal.pone.0177605. eCollection 2017. PLoS One. 2017. PMID: 28520810 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous