Gene expression profile in human leukocytes - PubMed (original) (raw)
Comparative Study
. 2003 May 1;101(9):3509-13.
doi: 10.1182/blood-2002-06-1866. Epub 2003 Jan 9.
Shigenori Nagai, Jun Sese, Takuji Suzuki, Aya Obata, Taku Sato, Nobuaki Toyoda, Hong-Yan Dong, Makoto Kurachi, Tomoyuki Nagahata, Ken-ichi Shizuno, Shinichi Morishita, Kouji Matsushima
Affiliations
- PMID: 12522010
- DOI: 10.1182/blood-2002-06-1866
Free article
Comparative Study
Gene expression profile in human leukocytes
Shin-ichi Hashimoto et al. Blood. 2003.
Free article
Abstract
Leukocytes are classified as myelocytic or lymphocytic, and each class of leukocytes consists of several types of cells that have different phenotypes and different roles. To define the gene expression in these cells, we have performed serial analysis of gene expression (SAGE) using human leukocytes and have provided the gene database for these cells not only at the resting stage but also at the activated stage. A total of 709,990 tags from 17 libraries were analyzed for the manifestation of gene expression profiles in various types of human leukocytes. Types of leukocytes analyzed were as follows: peripheral blood monocytes, colony-stimulating factor-induced macrophages, monocyte-derived immature dendritic cells, mature/activated dendritic cells, granulocytes, natural killer (NK) cells, resting B cells, activated B cells, naive T cells, CCR4(-) memory T cells (resting T(H)1 cells), CCR4(+) memory T cells (resting T(H)2 cells), activated T(H)1 cells, and activated T(H)2 cells. Among 38,961 distinct tags that appeared more than once in the combined total libraries, 27,323 tags were found to represent unique genes in certain type(s) of leukocytes. Using probability (P) and hierarchical clustering analysis, we identified the genes selectively expressed in each type of leukocytes. Identification of the genes specifically expressed in different types of leukocytes provides not only a novel molecular signature to define different subsets of resting and activated cells but also contributes to further understanding of the biologic function of leukocytes in the host defense system.
Similar articles
- Cell-type specific gene expression profiles of leukocytes in human peripheral blood.
Palmer C, Diehn M, Alizadeh AA, Brown PO. Palmer C, et al. BMC Genomics. 2006 May 16;7:115. doi: 10.1186/1471-2164-7-115. BMC Genomics. 2006. PMID: 16704732 Free PMC article. - Comprehensive gene expression analysis of human NK cells and CD8(+) T lymphocytes.
Obata-Onai A, Hashimoto S, Onai N, Kurachi M, Nagai S, Shizuno K, Nagahata T, Matsushima K. Obata-Onai A, et al. Int Immunol. 2002 Oct;14(10):1085-98. doi: 10.1093/intimm/dxf086. Int Immunol. 2002. PMID: 12356674 - Genetic approach to insight into the immunobiology of human dendritic cells and identification of CD84-H1, a novel CD84 homologue.
Zhang W, Wan T, Li N, Yuan Z, He L, Zhu X, Yu M, Cao X. Zhang W, et al. Clin Cancer Res. 2001 Mar;7(3 Suppl):822s-829s. Clin Cancer Res. 2001. PMID: 11300479 - A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells.
Colonna M, Nakajima H, Navarro F, López-Botet M. Colonna M, et al. J Leukoc Biol. 1999 Sep;66(3):375-81. doi: 10.1002/jlb.66.3.375. J Leukoc Biol. 1999. PMID: 10496306 Review. - Monocytes and dendritic cells in a hypoxic environment: Spotlights on chemotaxis and migration.
Bosco MC, Puppo M, Blengio F, Fraone T, Cappello P, Giovarelli M, Varesio L. Bosco MC, et al. Immunobiology. 2008;213(9-10):733-49. doi: 10.1016/j.imbio.2008.07.031. Epub 2008 Sep 21. Immunobiology. 2008. PMID: 18926289 Review.
Cited by
- The gene expression patterns of peripheral blood mononuclear cells in patients with systemic lupus erythematosus.
Li S, Jiang W, Huang R, Wang X, Liu W, Shen S. Li S, et al. J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):367-71. doi: 10.1007/s11596-007-0405-6. J Huazhong Univ Sci Technolog Med Sci. 2007. PMID: 17828488 - SAGE is far more sensitive than EST for detecting low-abundance transcripts.
Sun M, Zhou G, Lee S, Chen J, Shi RZ, Wang SM. Sun M, et al. BMC Genomics. 2004 Jan 5;5(1):1. doi: 10.1186/1471-2164-5-1. BMC Genomics. 2004. PMID: 14704093 Free PMC article. - 5'SAGE: 5'-end Serial Analysis of Gene Expression database.
Kasai Y, Hashimoto S, Yamada T, Sese J, Sugano S, Matsushima K, Morishita S. Kasai Y, et al. Nucleic Acids Res. 2005 Jan 1;33(Database issue):D550-2. doi: 10.1093/nar/gki085. Nucleic Acids Res. 2005. PMID: 15608259 Free PMC article. - Evaluation of an RNAseq-Based Immunogenomic Liquid Biopsy Approach in Early-Stage Prostate Cancer.
Van Neste L, Wojno KJ, Henao R, Mane S, Korman H, Hafron J, Kernen K, Tinawi-Aljundi R, Putzi M, Kassis AI, Kantoff PW. Van Neste L, et al. Cells. 2021 Sep 28;10(10):2567. doi: 10.3390/cells10102567. Cells. 2021. PMID: 34685549 Free PMC article. - SAGE analysis demonstrates increased expression of TOSO contributing to Fas-mediated resistance in CLL.
Proto-Siqueira R, Panepucci RA, Careta FP, Lee A, Clear A, Morris K, Owen C, Rizzatti EG, Silva WA Jr, Falcão RP, Zago MA, Gribben JG. Proto-Siqueira R, et al. Blood. 2008 Jul 15;112(2):394-7. doi: 10.1182/blood-2007-11-124065. Epub 2008 Apr 23. Blood. 2008. PMID: 18434611 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources