Bcl-2 and Bax modulate adenine nucleotide translocase activity - PubMed (original) (raw)
. 2003 Jan 15;63(2):541-6.
Helena L A Vieira, Florence Verrier, Grégoire Vandecasteele, Isabelle Cohen, Marie-Christine Prévost, Eric Larquet, Fabrizio Pariselli, Patrice X Petit, Axel Kahn, Rosario Rizzuto, Catherine Brenner, Guido Kroemer
Affiliations
- PMID: 12543814
Bcl-2 and Bax modulate adenine nucleotide translocase activity
Anne-Sophie Belzacq et al. Cancer Res. 2003.
Abstract
Bcl-2 is a prosurvival factor that reportedly prevents the nonspecific permeabilization of mitochondrial membranes, yet enhances specific ADP/ATP exchange by these organelles. Here, we show that Bcl-2 enhances the ADP/ATP exchange in proteoliposomes containing the purified adenine nucleotide translocase (ANT) in isolated mitochondria and mitoplasts, as well as in intact cells in which mitochondrial matrix ATP was monitored continuously using a specific luciferase-based assay system. Conversely, Bax, which displaces Bcl-2 from ANT in apoptotic cells, inhibits ADP/ATP exchange through a direct action on ANT. The Bax-mediated inhibition of ADP/ATP exchange can be separated from Bax-stimulated formation of nonspecific pores by ANT. Chemotherapy-induced apoptosis caused an inhibition of ANT activity, which preceded the loss of the mitochondrial transmembrane potential and could be prevented by overexpression of Bcl-2. These data are compatible with a model of mitochondrial apoptosis regulation in which ANT interacts with either Bax or Bcl-2, which both influence ANT function in opposing manners. Bcl-2 would maintain the translocase activity at high levels, whereas Bax would inhibit the translocase function of ANT.
Similar articles
- Bax and Bcl-xL independently regulate apoptotic changes of yeast mitochondria that require VDAC but not adenine nucleotide translocator.
Shimizu S, Shinohara Y, Tsujimoto Y. Shimizu S, et al. Oncogene. 2000 Sep 7;19(38):4309-18. doi: 10.1038/sj.onc.1203788. Oncogene. 2000. PMID: 10980606 - Bcl-2 and Bax regulate the channel activity of the mitochondrial adenine nucleotide translocator.
Brenner C, Cadiou H, Vieira HL, Zamzami N, Marzo I, Xie Z, Leber B, Andrews D, Duclohier H, Reed JC, Kroemer G. Brenner C, et al. Oncogene. 2000 Jan 20;19(3):329-36. doi: 10.1038/sj.onc.1203298. Oncogene. 2000. PMID: 10656679 - Cell permeable BH3-peptides overcome the cytoprotective effect of Bcl-2 and Bcl-X(L).
Vieira HL, Boya P, Cohen I, El Hamel C, Haouzi D, Druillenec S, Belzacq AS, Brenner C, Roques B, Kroemer G. Vieira HL, et al. Oncogene. 2002 Mar 27;21(13):1963-77. doi: 10.1038/sj.onc.1205270. Oncogene. 2002. PMID: 11960369 - Permeabilization of the mitochondrial inner membrane during apoptosis: impact of the adenine nucleotide translocator.
Vieira HL, Haouzi D, El Hamel C, Jacotot E, Belzacq AS, Brenner C, Kroemer G. Vieira HL, et al. Cell Death Differ. 2000 Dec;7(12):1146-54. doi: 10.1038/sj.cdd.4400778. Cell Death Differ. 2000. PMID: 11175251 Review.
Cited by
- The Basis and Advances in Clinical Application of Cytomegalovirus-Specific Cytotoxic T Cell Immunotherapy for Glioblastoma Multiforme.
Daei Sorkhabi A, Sarkesh A, Saeedi H, Marofi F, Ghaebi M, Silvestris N, Baradaran B, Brunetti O. Daei Sorkhabi A, et al. Front Oncol. 2022 Apr 19;12:818447. doi: 10.3389/fonc.2022.818447. eCollection 2022. Front Oncol. 2022. PMID: 35515137 Free PMC article. Review. - Zika virus infection induced apoptosis by modulating the recruitment and activation of pro-apoptotic protein Bax.
Han X, Wang J, Yang Y, Qu S, Wan F, Zhang Z, Wang R, Li G, Cong H. Han X, et al. J Virol. 2021 Mar 25;95(8):e01445-20. doi: 10.1128/JVI.01445-20. Epub 2021 Feb 3. J Virol. 2021. PMID: 33536166 Free PMC article. - Regulation of Cell Death by Mitochondrial Transport Systems of Calcium and Bcl-2 Proteins.
Naumova N, Šachl R. Naumova N, et al. Membranes (Basel). 2020 Oct 21;10(10):299. doi: 10.3390/membranes10100299. Membranes (Basel). 2020. PMID: 33096926 Free PMC article. Review. - Regulation of the mitochondrial permeability transition pore and its effects on aging.
Pellegrino-Coppola D. Pellegrino-Coppola D. Microb Cell. 2020 Jun 22;7(9):222-233. doi: 10.15698/mic2020.09.728. Microb Cell. 2020. PMID: 32904375 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials