Distinct neurochemical mechanisms are activated following administration of different P2X receptor agonists into the hindpaw of a rat - PubMed (original) (raw)
Distinct neurochemical mechanisms are activated following administration of different P2X receptor agonists into the hindpaw of a rat
Carol T Wismer et al. Brain Res. 2003.
Abstract
Nocifensive behaviors induced by the intradermal injection of three different P2X receptor agonists, ATP, BzATP or alpha,beta-meATP, into a hindpaw were measured in rats that were injected intrathecally with either an NMDA (MK-801) or an NK-1 (L-703,606) receptor antagonist or were pretreated systemically with the VR1 agonist resiniferatoxin (RTX). The same procedures were performed in animals injected intradermally with either capsaicin or formalin. Spinal infusion of MK-801 (10-50 nmol/10 micro l) similarly reduced the number of nociceptive events triggered by each of the P2X agonists and was also effective against capsaicin and formalin induced behaviors. Intrathecal administration of L-703,606 (50-100 nmol/10 micro l) had its greatest antinociceptive effect against capsaicin-induced behaviors followed by ATP and BzATP. L-703,606 was completely ineffective against behaviors induced by formalin or the other P2X agonist, alpha,beta-meATP. Pretreatment with RTX 2 days prior to testing significantly decreased the number of nociceptive events caused by each of the P2X agonists as well as capsaicin and formalin (capsaicin>BzATP>ATP>formalin>alpha,beta-meATP). The remaining nociceptive events in RTX animals injected with alpha,beta-meATP were significantly higher than in animals injected with either ATP or BzATP. Intradermal administration of different P2X receptor agonists induced similar levels of nocifensive behaviors and activity at spinal NMDA receptors. Capsaicin-sensitive fibers were likely activated following injection of BzATP and ATP, but not alpha,beta-meATP, and appeared to trigger the spinal release of substance P. The differences in mechanisms employed by the different P2X agonists may be a function of respective selectivity for P2X receptor subtypes.
Similar articles
- Mechanical allodynia caused by intraplantar injection of P2X receptor agonist in rats: involvement of heteromeric P2X2/3 receptor signaling in capsaicin-insensitive primary afferent neurons.
Tsuda M, Koizumi S, Kita A, Shigemoto Y, Ueno S, Inoue K. Tsuda M, et al. J Neurosci. 2000 Aug 1;20(15):RC90. doi: 10.1523/JNEUROSCI.20-15-j0007.2000. J Neurosci. 2000. PMID: 10899177 Free PMC article. - Modulation of BzATP and formalin induced nociception: attenuation by the P2X receptor antagonist, TNP-ATP and enhancement by the P2X(3) allosteric modulator, cibacron blue.
Jarvis MF, Wismer CT, Schweitzer E, Yu H, van Biesen T, Lynch KJ, Burgard EC, Kowaluk EA. Jarvis MF, et al. Br J Pharmacol. 2001 Jan;132(1):259-69. doi: 10.1038/sj.bjp.0703793. Br J Pharmacol. 2001. PMID: 11156585 Free PMC article. - Effect of tetramethylpyrazine on acute nociception mediated by signaling of P2X receptor activation in rat.
Liang SD, Gao Y, Xu CS, Xu BH, Mu SN. Liang SD, et al. Brain Res. 2004 Jan 9;995(2):247-52. doi: 10.1016/j.brainres.2003.09.070. Brain Res. 2004. PMID: 14672814 - Acute nociception mediated by hindpaw P2X receptor activation in the rat.
Bland-Ward PA, Humphrey PP. Bland-Ward PA, et al. Br J Pharmacol. 1997 Sep;122(2):365-71. doi: 10.1038/sj.bjp.0701371. Br J Pharmacol. 1997. PMID: 9313948 Free PMC article. - P2X purinoceptor-mediated excitation of trigeminal lingual nerve terminals in an in vitro intra-arterially perfused rat tongue preparation.
Rong W, Burnstock G, Spyer KM. Rong W, et al. J Physiol. 2000 May 1;524 Pt 3(Pt 3):891-902. doi: 10.1111/j.1469-7793.2000.00891.x. J Physiol. 2000. PMID: 10790166 Free PMC article.
Cited by
- Crossing the pain barrier: P2 receptors as targets for novel analgesics.
Kennedy C, Assis TS, Currie AJ, Rowan EG. Kennedy C, et al. J Physiol. 2003 Dec 15;553(Pt 3):683-94. doi: 10.1113/jphysiol.2003.049114. Epub 2003 Sep 26. J Physiol. 2003. PMID: 14514872 Free PMC article. Review. - Translocation of neuronal nitric oxide synthase to the plasma membrane by ATP is mediated by P2X and P2Y receptors.
Ohnishi T, Matsumura S, Ito S. Ohnishi T, et al. Mol Pain. 2009 Jul 20;5:40. doi: 10.1186/1744-8069-5-40. Mol Pain. 2009. PMID: 19619286 Free PMC article. - Effects of A-317491, a novel and selective P2X3/P2X2/3 receptor antagonist, on neuropathic, inflammatory and chemogenic nociception following intrathecal and intraplantar administration.
McGaraughty S, Wismer CT, Zhu CZ, Mikusa J, Honore P, Chu KL, Lee CH, Faltynek CR, Jarvis MF. McGaraughty S, et al. Br J Pharmacol. 2003 Dec;140(8):1381-8. doi: 10.1038/sj.bjp.0705574. Epub 2003 Nov 17. Br J Pharmacol. 2003. PMID: 14623769 Free PMC article. - Spinal astrocytes produce and secrete dynorphin neuropeptides.
Wahlert A, Funkelstein L, Fitzsimmons B, Yaksh T, Hook V. Wahlert A, et al. Neuropeptides. 2013 Apr;47(2):109-15. doi: 10.1016/j.npep.2012.10.006. Epub 2013 Jan 3. Neuropeptides. 2013. PMID: 23290538 Free PMC article. - P2X3 receptor involvement in pain states.
Wirkner K, Sperlagh B, Illes P. Wirkner K, et al. Mol Neurobiol. 2007 Oct;36(2):165-83. doi: 10.1007/s12035-007-0033-y. Epub 2007 Jul 17. Mol Neurobiol. 2007. PMID: 17952660 Review.