Microviscosity parameters and protein mobility in biological membranes - PubMed (original) (raw)
Microviscosity parameters and protein mobility in biological membranes
M Shinitzky et al. Biochim Biophys Acta. 1976.
Abstract
A fluorescence polarization technique with 1,6-diphenyl 1,3,5-hexatriene as a probe were employed to determine the microviscosity, n, in liposomes and biological membranes of different cholesterol to phospholipid mol ratio. From the temperature profile of n the flow activation energy, deltaE, and the unit flow volume, V, were derived. The increase of cholesterol/phospholipid ratio in liposomes is followed by a marked increase in n and a decrease in both deltaE and V. Liposomes of the same phospholipid composition as human erythrocyte membranes display in the extreme cases of cholesterol/phospholipid ratios 0 and 1.4 the values of n(25 degrees C) = 1.8 and 9.1 P, and deltaE = 15.0 and 6.5 kcal/mol, respectively. For most membranes studied the fluorescence polarization characteristics and the corresponding n values are similar to those obtained with these liposomes when the cholesterol/phospholipid level of the liposomes and the membranes were the same. However, unlike in liposomes deltaE of all membranes is in the narrow range of 6.5-8.5 kcal/mol, regardless of its cholesterol/phospholipid level. It is plausible that this is a general characteristic of biological membranes which originates from the vertical movement of membrane proteins to an equilibrium position which maintains constant deltaE and V values. This type of movement should affect the interrelation between lipid fluidity and protein mobility. Lipid microviscosity and the degree of rotational mobility of concanavalin A receptor sites in cell membranes were therefore determined. The examined cells were normal and malignant fibroblasts, as an example of cells that form solid tumours in vivo, and normal and malignant lymphocytes, as an example of cells that form ascites tumours in vivo. In both cell systems, opposite correlations between the lipid fluidity and the mobility of concanavalin A receptors were observed. In the fibroblasts the malignant cells possess a lower lipid fluidity but a higher receptor mobility, whereas in the lymphocytes the malignant cells possess a higher lipid fluidity but a lower receptor mobility. Thus, in these cell systems the degree of rotational mobility of concanavalin A receptors increases upon decreasing the lipid fluidity and decreases upon increasing the fluidity of the lipid core. This dynamic feature is in line with the above proposal according to which the concanavalin A receptor sites become more exposed to the aqueous surrounding upon increasing the microviscosity of the lipid layer and vice versa.
Similar articles
- Membrane microviscosity and human platelet function.
Shattil SJ, Cooper RA. Shattil SJ, et al. Biochemistry. 1976 Nov 2;15(22):4832-7. doi: 10.1021/bi00667a012. Biochemistry. 1976. PMID: 990246 - Modulation of the binding and endocytosis of concanavalin A by guinea pig keratinocytes: reversible antagonistic effects of cholesterol and phospholipid-liposomes.
Callaghan TM, Metezeau P, Gachelin H, Redziniak G, Milner Y, Goldberg ME. Callaghan TM, et al. J Invest Dermatol. 1990 Jan;94(1):58-64. doi: 10.1111/1523-1747.ep12873359. J Invest Dermatol. 1990. PMID: 2295838 - Fluidity of natural membranes and phosphatidylserine and ganglioside dispersions. Effect of local anesthetics, cholesterol and protein.
Feinstein MB, Fernandez SM, Sha'afi RI. Feinstein MB, et al. Biochim Biophys Acta. 1975 Dec 16;413(3):354-70. doi: 10.1016/0005-2736(75)90121-2. Biochim Biophys Acta. 1975. PMID: 1191696 - Dynamic structure of biological and model membranes: analysis by optical anisotropy decay measurement.
Kinosita K Jr, Kawato S, Ikegami A. Kinosita K Jr, et al. Adv Biophys. 1984;17:147-203. doi: 10.1016/0065-227x(84)90027-3. Adv Biophys. 1984. PMID: 6399815 Review. - Protein-liposome interactions and their relevance to the structure and function of cell membranes.
Kimelberg HK. Kimelberg HK. Mol Cell Biochem. 1976 Feb 25;10(3):171-90. doi: 10.1007/BF01731688. Mol Cell Biochem. 1976. PMID: 177856 Review.
Cited by
- Lipidome Atlas of the Developing Heart Uncovers Dynamic Membrane Lipid Attributes Underlying Cardiac Structural and Metabolic Maturation.
Miao H, Li B, Wang Z, Mu J, Tian Y, Jiang B, Zhang S, Gong X, Shui G, Lam SM. Miao H, et al. Research (Wash D C). 2022 Dec 19;2022:0006. doi: 10.34133/research.0006. eCollection 2022. Research (Wash D C). 2022. PMID: 39290970 Free PMC article. - Association between the change of total cholesterol during adolescence and depressive symptoms in early adulthood.
Park JH, Jung SJ, Jung Y, Ahn SV, Lee E, Kim HC. Park JH, et al. Eur Child Adolesc Psychiatry. 2021 Feb;30(2):261-269. doi: 10.1007/s00787-020-01511-w. Epub 2020 Mar 19. Eur Child Adolesc Psychiatry. 2021. PMID: 32193646 - Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence.
Panagopoulou MS, Wark AW, Birch DJS, Gregory CD. Panagopoulou MS, et al. J Extracell Vesicles. 2020 Jan 7;9(1):1710020. doi: 10.1080/20013078.2019.1710020. eCollection 2020. J Extracell Vesicles. 2020. PMID: 32002172 Free PMC article. Review. - Forced Unfolding Mechanism of Bacteriorhodopsin as Revealed by Coarse-Grained Molecular Dynamics.
Yamada T, Yamato T, Mitaku S. Yamada T, et al. Biophys J. 2016 Nov 15;111(10):2086-2098. doi: 10.1016/j.bpj.2016.09.051. Biophys J. 2016. PMID: 27851934 Free PMC article. - There Is No Simple Model of the Plasma Membrane Organization.
Bernardino de la Serna J, Schütz GJ, Eggeling C, Cebecauer M. Bernardino de la Serna J, et al. Front Cell Dev Biol. 2016 Sep 29;4:106. doi: 10.3389/fcell.2016.00106. eCollection 2016. Front Cell Dev Biol. 2016. PMID: 27747212 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources