Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells - PubMed (original) (raw)
Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells
E D Salmon et al. J Cell Biol. 1976 May.
Abstract
Evidence from light microscopy (principally polarization microscopy) has demonstrated that hydrostatic pressure can reversibly inhibit mitosis by rapidly depolymerizing the spindle fiber microtubules. We have confirmed this finding in ultrastructural studies of mitotic HeLa cells incubated at 37 degrees C and pressurized at 680 atm (10,000 psi). Althouth there are many spindle microtubules in the cells at atmospheric pressure, electron micographs of cells pressurized for 10 min (and fixed while under pressure in a Landau-Thibodeau chamber) show few microtubules. Pressure has a differential effect on the various types of spindle microtubules. Astral and interpolar MTs appear to be completely depolymerized in pressurized cells, but occasional groups of kinetochore fiber microtubules are seen. Surprisingly, the length and density of microtubules of the stem bodies and midbody of telophase cells appear unchanged by pressurization. In cells fixed 10 min after pressure was released, microtubules were again abundant, the density often appearing to be higher than in control cells. Reorganization seems incomplete, however, since many of the microtubules are randomly oriented. Unexpectedly, kinetochores appeared diffuse and were difficult to identify in sections of pressurized cells. Even after 10 min of recovery at atmospheric pressure, their structure was less distinct than in unpressurized cells.
Similar articles
- Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement.
Salmon ED. Salmon ED. Ann N Y Acad Sci. 1975 Jun 30;253:383-406. doi: 10.1111/j.1749-6632.1975.tb19216.x. Ann N Y Acad Sci. 1975. PMID: 1096721 Review. - Dynamics of spindle microtubule organization: kinetochore fiber microtubules of plant endosperm.
Jensen CG. Jensen CG. J Cell Biol. 1982 Feb;92(2):540-58. doi: 10.1083/jcb.92.2.540. J Cell Biol. 1982. PMID: 7061596 Free PMC article. - Mitosis in Oedogonium: spindle microfilaments and the origin of the kinetochore fiber.
Schibler MJ, Pickett-Heaps JD. Schibler MJ, et al. Eur J Cell Biol. 1980 Oct;22(2):687-98. Eur J Cell Biol. 1980. PMID: 7192627 - Cytochalasin J affects chromosome congression and spindle microtubule organization in PtK1 cells.
Snyder JA, Cohen L. Snyder JA, et al. Cell Motil Cytoskeleton. 1995;32(4):245-57. doi: 10.1002/cm.970320402. Cell Motil Cytoskeleton. 1995. PMID: 8608604 - Motile kinetochores and polar ejection forces dictate chromosome position on the vertebrate mitotic spindle.
Rieder CL, Salmon ED. Rieder CL, et al. J Cell Biol. 1994 Feb;124(3):223-33. doi: 10.1083/jcb.124.3.223. J Cell Biol. 1994. PMID: 8294508 Free PMC article. Review.
Cited by
- Kinesin-like protein CHO1 is required for the formation of midbody matrix and the completion of cytokinesis in mammalian cells.
Matuliene J, Kuriyama R. Matuliene J, et al. Mol Biol Cell. 2002 Jun;13(6):1832-45. doi: 10.1091/mbc.01-10-0504. Mol Biol Cell. 2002. PMID: 12058052 Free PMC article. - Self-organization of kinetochore-fibers in human mitotic spindles.
Conway W, Kiewisz R, Fabig G, Kelleher CP, Wu HY, Anjur-Dietrich M, Müller-Reichert T, Needleman DJ. Conway W, et al. Elife. 2022 Jul 25;11:e75458. doi: 10.7554/eLife.75458. Elife. 2022. PMID: 35876665 Free PMC article. - Cytoskeletal and muscle-like elements in cochlear hair cells.
Zenner HP. Zenner HP. Arch Otorhinolaryngol. 1981;230(1):81-92. doi: 10.1007/BF00665383. Arch Otorhinolaryngol. 1981. PMID: 7011285 - Biophysics of mitosis.
McIntosh JR, Molodtsov MI, Ataullakhanov FI. McIntosh JR, et al. Q Rev Biophys. 2012 May;45(2):147-207. doi: 10.1017/S0033583512000017. Epub 2012 Feb 10. Q Rev Biophys. 2012. PMID: 22321376 Free PMC article. Review. - Intact microtubules are required for rapid turnover of carboxyl-terminal tyrosine of alpha-tubulin in cell cultures.
Thompson WC, Deanin GG, Gordon MW. Thompson WC, et al. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1318-22. doi: 10.1073/pnas.76.3.1318. Proc Natl Acad Sci U S A. 1979. PMID: 286314 Free PMC article.
References
- Soc Gen Physiol Ser. 1975;30:31-76 - PubMed
- Biophys J. 1975 Jul;15(7):725-44 - PubMed
- J Cell Biol. 1975 Jul;66(1):114-27 - PubMed
- Soc Gen Physiol Ser. 1975;30:181-206 - PubMed
- Chromosoma. 1973;40(1):43-82 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous