Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling - PubMed (original) (raw)
. 2003 May 16;278(20):18321-9.
doi: 10.1074/jbc.M211541200. Epub 2003 Mar 11.
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- PMID: 12637562
- DOI: 10.1074/jbc.M211541200
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Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signaling
Ricardo P Souto et al. J Biol Chem. 2003.
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Abstract
Adipocytes play an important role in the insulin-dependent regulation of organismal fuel metabolism and express caveolae at levels as high or higher than any other cell type. Recently, a link between insulin signaling and caveolae has been suggested; nevertheless, adipocyte caveolae have been the subject of relatively few studies, and their contents have been minimally characterized. With the aid of a new monoclonal antibody, we developed a rapid procedure for the immunoisolation of caveolae derived from the plasma membrane of adipocytes, and we characterized their protein content. We find that immunopurified adipocyte caveolae have a relatively limited protein composition, and they lack the raft protein, flotillin, and insulin receptors. Immunogold labeling and electron microscopy of the adipocyte plasma membrane confirmed the lack of insulin receptors in caveolae. In addition to caveolins, the structural components of caveolae, their major protein constituents, are the semicarbazide-sensitive amine oxidase and the scavenger lipoprotein receptor CD36. The results are consistent with a role for caveolae in lipid flux in and of adipocytes.
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