Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env - PubMed (original) (raw)

. 2003 Jul 1;102(1):223-8.

doi: 10.1182/blood-2003-01-0110. Epub 2003 Mar 20.

Yuka Hoshino, Yoshihiko Toda, Shizunobu Igimi, Yoshitsugu Kojima, Nao Jounai, Kenji Ohba, Akira Kushiro, Mayumi Kiwaki, Kenji Hamajima, Dennis Klinman, Kenji Okuda

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Immunogenicity and protective efficacy of orally administered recombinant Lactococcus lactis expressing surface-bound HIV Env

Ke-Qin Xin et al. Blood. 2003.

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Abstract

This study investigates whether genetically modified orally administered Lactococcus lactis (L lactis) could be used as an HIV vaccine. L lactis is immunogenic and extremely safe when delivered orally. We created a recombinant L lactis vector expressing the envelope protein of HIV on its cell surface. Oral immunization with this vector induced high levels of HIV-specific serum IgG and fecal IgA antibodies. Cell-mediated immune responses also were generated in both the regional lymph nodes and the spleen. Dendritic cells are readily infected by L lactis and appear to play a potential role in mediating the development of these immune responses. The protective efficacy of this vaccine strategy was demonstrated by challenging mice intraperitoneally with an HIV Env-expressing vaccinia virus. Their viral loads were 350-fold lower than those of control mice. These findings support the further development of L lactis-based HIV vaccines.

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