Cu,Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis - PubMed (original) (raw)

Cu,Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis

Adriana Garay-Arroyo et al. FEBS Lett. 2003.

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Abstract

Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn-superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N-acetylcysteine to the medium. Pretreatment with MnCl(2), a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A-cAMP and SKN7 pathways.

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