Different Ca2+ dynamics between isolated hippocampal pyramidal cells and dentate granule cells - PubMed (original) (raw)
Different Ca2+ dynamics between isolated hippocampal pyramidal cells and dentate granule cells
Atsushi Baba et al. J Neurocytol. 2002 Jan.
Abstract
The hippocampal formation contains a variety of neuronal types. The principal neurons are granule cells in the dentate gyrus and pyramidal cells in Ammon's horn. These two neuron types show distinct cell morphology and display a different vulnerability to ischemic injury or various neurotoxins. In order to illustrate the difference in the pathophysiological properties of these neurons, we established a method for separately culturing granule cells and pyramidal cells. They were prepared from the dentate gyrus and Ammon's horn of 3-day-old Wistar rat pups and maintained for 7-9 days in culture. After transient exposure to N-methyl-D-aspartate or glutamate, both the cultured neuron populations displayed somatic Ca(2+) transients with similar amplitudes, but the subsequent recovery to baseline was about twice as fast in granule cells than in pyramidal cells. Similar results were obtained for K(+) depolarization-induced Ca(2+) elevation, suggesting that the relatively rapid Ca(2+) clearance in granule cells is independent of Ca(2+) influx pathways. The present study provides the first evidence for a difference in Ca(2+) dynamics and homeostasis between granule and pyramidal cells and may represent a cellular basis for the differential vulnerability of hippocampal neurons.
Similar articles
- Cellular and Molecular Differences Between Area CA1 and the Dentate Gyrus of the Hippocampus.
Alkadhi KA. Alkadhi KA. Mol Neurobiol. 2019 Sep;56(9):6566-6580. doi: 10.1007/s12035-019-1541-2. Epub 2019 Mar 14. Mol Neurobiol. 2019. PMID: 30874972 Review. - Distribution, morphological features, and synaptic connections of parvalbumin- and calbindin D28k-immunoreactive neurons in the human hippocampal formation.
Seress L, Gulyás AI, Ferrer I, Tunon T, Soriano E, Freund TF. Seress L, et al. J Comp Neurol. 1993 Nov 8;337(2):208-30. doi: 10.1002/cne.903370204. J Comp Neurol. 1993. PMID: 8276998 - Cell formation in the human hippocampal formation from mid-gestation to the late postnatal period.
Seress L, Abrahám H, Tornóczky T, Kosztolányi G. Seress L, et al. Neuroscience. 2001;105(4):831-43. doi: 10.1016/s0306-4522(01)00156-7. Neuroscience. 2001. PMID: 11530221 - Decreased expression and functionality of NMDA receptor complexes persist in the CA1, but not in the dentate gyrus after transient cerebral ischemia.
Hsu JC, Zhang Y, Takagi N, Gurd JW, Wallace MC, Zhang L, Eubanks JH. Hsu JC, et al. J Cereb Blood Flow Metab. 1998 Jul;18(7):768-75. doi: 10.1097/00004647-199807000-00008. J Cereb Blood Flow Metab. 1998. PMID: 9663507 - Intrinsic factors in the selective vulnerability of hippocampal pyramidal neurons.
Mattson MP, Guthrie PB, Kater SB. Mattson MP, et al. Prog Clin Biol Res. 1989;317:333-51. Prog Clin Biol Res. 1989. PMID: 2690106 Review.
Cited by
- Cellular and Molecular Differences Between Area CA1 and the Dentate Gyrus of the Hippocampus.
Alkadhi KA. Alkadhi KA. Mol Neurobiol. 2019 Sep;56(9):6566-6580. doi: 10.1007/s12035-019-1541-2. Epub 2019 Mar 14. Mol Neurobiol. 2019. PMID: 30874972 Review. - Comparison of the Effect of Exercise on Late-Phase LTP of the Dentate Gyrus and CA1 of Alzheimer's Disease Model.
Dao AT, Zagaar MA, Levine AT, Alkadhi KA. Dao AT, et al. Mol Neurobiol. 2016 Dec;53(10):6859-6868. doi: 10.1007/s12035-015-9612-5. Epub 2015 Dec 12. Mol Neurobiol. 2016. PMID: 26660327 - Differentiation of apical and basal dendrites in pyramidal cells and granule cells in dissociated hippocampal cultures.
Wu YK, Fujishima K, Kengaku M. Wu YK, et al. PLoS One. 2015 Feb 23;10(2):e0118482. doi: 10.1371/journal.pone.0118482. eCollection 2015. PLoS One. 2015. PMID: 25705877 Free PMC article. - Long-range axonal calcium sweep induces axon retraction.
Yamada RX, Sasaki T, Ichikawa J, Koyama R, Matsuki N, Ikegaya Y. Yamada RX, et al. J Neurosci. 2008 Apr 30;28(18):4613-8. doi: 10.1523/JNEUROSCI.0019-08.2008. J Neurosci. 2008. PMID: 18448637 Free PMC article. - Activity-evoked capacitative Ca2+ entry: implications in synaptic plasticity.
Baba A, Yasui T, Fujisawa S, Yamada RX, Yamada MK, Nishiyama N, Matsuki N, Ikegaya Y. Baba A, et al. J Neurosci. 2003 Aug 27;23(21):7737-41. doi: 10.1523/JNEUROSCI.23-21-07737.2003. J Neurosci. 2003. PMID: 12944501 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous