Multiplex PCR for detection of aminoglycoside resistance genes in enterococci - PubMed (original) (raw)

Multiplex PCR for detection of aminoglycoside resistance genes in enterococci

Sergei B Vakulenko et al. Antimicrob Agents Chemother. 2003 Apr.

Abstract

A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2")-Ia, aph(2")-Ib, aph(2")-Ic, aph(2")-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.

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Figures

FIG. 1.

FIG. 1.

Agarose gel electrophoresis of multiplex PCR products from enterococcal isolates tested for six aminoglycoside resistance genes: aac(6′)-Ie-aph(2")-Ia (348 bp), aph(2")-Ib (867 bp), aph(2")-Ic (444 bp), aph(2")-Id (641 bp), aph(3′)-IIIa (523 bp), and ant(4′)-Ia (294 bp). Lane 1, positive control from a single reaction with PCR products from three isolates that together contain the six genes studied (see text). Lanes 2 to 7, six representative isolates, respectively, that possess one or more of the six genes studied.

References

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