Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses - PubMed (original) (raw)

Comparative Study

. 2003 Apr;84(Pt 4):789-793.

doi: 10.1099/vir.0.18963-0.

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Comparative Study

Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses

Alain Kohl et al. J Gen Virol. 2003 Apr.

Abstract

The genome of Bunyamwera virus (BUN) consists of three segments of single-stranded RNA of negative polarity. The smallest segment, S, encodes the N protein and a nonstructural protein called NSs. We recently described a mutant virus (BUNdelNSs) that does not express NSs but overexpresses N and grows to lower titres than wild-type (wt) BUN. Here we report a BUNdelNSs variant that expresses lower levels of N protein and grows to higher titres. Sequencing of the 3' and 5' termini of the BUNdelNSs S RNA segment and analysis using a minireplicon system show that the N overexpressing phenotype results from a single nucleotide substitution at position 16 in the 3' terminus. This mutation could also be detected in wtBUN populations, and was isolated by plaquing a 'wt' variant carrying the mutation. This variant was found to express increased N and NSs levels, and grew to lower titres than wtBUN.

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