Gene expression profiling of plasma cells and plasmablasts: toward a better understanding of the late stages of B-cell differentiation - PubMed (original) (raw)
Comparative Study
. 2003 Jul 15;102(2):592-600.
doi: 10.1182/blood-2002-10-3161. Epub 2003 Mar 27.
Affiliations
- PMID: 12663452
- DOI: 10.1182/blood-2002-10-3161
Free article
Comparative Study
Gene expression profiling of plasma cells and plasmablasts: toward a better understanding of the late stages of B-cell differentiation
Karin Tarte et al. Blood. 2003.
Free article
Abstract
Plasma cells (PCs), the end point of B-cell differentiation, are a heterogeneous cell compartment comprising several cell subsets from short-lived highly proliferative plasmablasts to long-lived nondividing fully mature PCs. Whereas the major transcription factors driving the differentiation of B cells to PCs were recently identified, the subtle genetic changes that underlie the transition from plasmablasts to mature PCs are poorly understood. We recently described an in vitro model making it possible to obtain a large number of cells with the morphologic, phenotypic, and functional characteristics of normal polyclonal plasmablastic cells (PPCs). Using Affymetrix microarrays we compared the gene expression profiles of these PPCs with those of mature PCs isolated from tonsils (TPCs) and bone marrow (BMPCs), and with those of B cells purified from peripheral blood (PBB cells) and tonsils (TBCs). Unsupervised principal component analysis clearly distinguished the 5 cell populations on the basis of their differentiation and proliferation status. Detailed statistical analysis allowed the identification of 85 PC genes and 40 B-cell genes, overexpressed, respectively, in the 3 PC subsets or in the 2 B-cell subsets. In addition, several signaling molecules and antiapoptotic proteins were found to be induced in BMPCs compared with PPCs and could be involved in the accumulation and prolonged survival of BMPCs in close contact with specialized stromal microenvironment. These data should help to better understand the molecular events that regulate commitment to a PC fate, mediate PC maintenance in survival niches, and could facilitate PC immortalization in plasma cell dyscrasias.
Similar articles
- An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization.
Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B. Jourdan M, et al. Blood. 2009 Dec 10;114(25):5173-81. doi: 10.1182/blood-2009-07-235960. Blood. 2009. PMID: 19846886 Free PMC article. - Generation of polyclonal plasmablasts from peripheral blood B cells: a normal counterpart of malignant plasmablasts.
Tarte K, De Vos J, Thykjaer T, Zhan F, Fiol G, Costes V, Rème T, Legouffe E, Rossi JF, Shaughnessy J Jr, Ørntoft TF, Klein B. Tarte K, et al. Blood. 2002 Aug 15;100(4):1113-22. Blood. 2002. PMID: 12149187 - Transcriptional Analysis of the Human IgE-Expressing Plasma Cell Differentiation Pathway.
Ramadani F, Bowen H, Gould HJ, Fear DJ. Ramadani F, et al. Front Immunol. 2019 Mar 11;10:402. doi: 10.3389/fimmu.2019.00402. eCollection 2019. Front Immunol. 2019. PMID: 30915071 Free PMC article. - Limited humoral immunoglobulin E memory influences serum immunoglobulin E levels in blood.
Achatz-Straussberger G, Zaborsky N, Königsberger S, Feichtner S, Lenz S, Peckl-Schmid D, Lamers M, Achatz G. Achatz-Straussberger G, et al. Clin Exp Allergy. 2009 Sep;39(9):1307-13. doi: 10.1111/j.1365-2222.2009.03278.x. Epub 2009 May 20. Clin Exp Allergy. 2009. PMID: 19489847 Free PMC article. Review. - Microarray-based understanding of normal and malignant plasma cells.
De Vos J, Hose D, Rème T, Tarte K, Moreaux J, Mahtouk K, Jourdan M, Goldschmidt H, Rossi JF, Cremer FW, Klein B. De Vos J, et al. Immunol Rev. 2006 Apr;210:86-104. doi: 10.1111/j.0105-2896.2006.00362.x. Immunol Rev. 2006. PMID: 16623766 Free PMC article. Review.
Cited by
- B cell heterogeneity in human tuberculosis highlights compartment-specific phenotype and functional roles.
Krause R, Ogongo P, Tezera L, Ahmed M, Mbano I, Chambers M, Ngoepe A, Magnoumba M, Muema D, Karim F, Khan K, Lumamba K, Nargan K, Madansein R, Steyn A, Shalek AK, Elkington P, Leslie A. Krause R, et al. Commun Biol. 2024 May 16;7(1):584. doi: 10.1038/s42003-024-06282-7. Commun Biol. 2024. PMID: 38755239 Free PMC article. - Enhancing prognostic power in multiple myeloma using a plasma cell signature derived from single-cell RNA sequencing.
Li JR, Arsang-Jang S, Cheng Y, Sun F, D'Souza A, Dhakal B, Hari P, Huang Q, Auer P, Li Y, Urrutia R, Zhan F, Shaughnessy JD Jr, Janz S, Dong J, Cheng C. Li JR, et al. Blood Cancer J. 2024 Mar 6;14(1):38. doi: 10.1038/s41408-024-01024-8. Blood Cancer J. 2024. PMID: 38443358 Free PMC article. - Majority of human circulating IgG plasmablasts stop blasting in a cell-free pro-survival culture.
Nguyen DC, Saney C, Hentenaar IT, Cabrera-Mora M, Capric V, Woodruff MC, Andrews J, Lonial S, Sanz I, Lee FE. Nguyen DC, et al. Sci Rep. 2024 Feb 13;14(1):3616. doi: 10.1038/s41598-024-53977-2. Sci Rep. 2024. PMID: 38350990 Free PMC article. - New insights into the ontogeny, diversity, maturation and survival of long-lived plasma cells.
Fooksman DR, Jing Z, Park R. Fooksman DR, et al. Nat Rev Immunol. 2024 Jul;24(7):461-470. doi: 10.1038/s41577-024-00991-0. Epub 2024 Feb 8. Nat Rev Immunol. 2024. PMID: 38332373 Review. - Predicting cell types with supervised contrastive learning on cells and their types.
Heryanto YD, Zhang YZ, Imoto S. Heryanto YD, et al. Sci Rep. 2024 Jan 3;14(1):430. doi: 10.1038/s41598-023-50185-2. Sci Rep. 2024. PMID: 38172501 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources