Identification of NF-kappa B-regulated genes induced by TNFalpha utilizing expression profiling and RNA interference - PubMed (original) (raw)
. 2003 Apr 3;22(13):2054-64.
doi: 10.1038/sj.onc.1206262.
Affiliations
- PMID: 12673210
- DOI: 10.1038/sj.onc.1206262
Identification of NF-kappa B-regulated genes induced by TNFalpha utilizing expression profiling and RNA interference
Anwu Zhou et al. Oncogene. 2003.
Erratum in
- Oncogene. 2004 Dec 16;23(58):9447
Abstract
Tumor necrosis factor alpha (TNF alpha) is a proinflammatory cytokine with important roles in regulating inflammatory responses as well as cell cycle proliferation and apoptosis. Although TNFalpha stimulates apoptosis, it also activates the transcription factor NF-kappa B, and studies have shown that inhibition of NF-kappa B potentiates the cytotoxicity of TNFalpha. Since several chemotherapy agents act like TNFalpha to both promote apoptosis and activate NF-kappa B, understanding the role of NF-kappa B in suppressing apoptosis may have significant clinical applications. To understand the effects of stimulation with TNFalpha and the role of NF-kappa B in regulating this response, a 23k human cDNA microarray was used to screen TNFalpha-inducible genes in HeLa cells. Real-time PCR verified expression changes in 16 of these genes and revealed three distinct temporal patterns of expression after TNFalpha stimulation. Using RNA interference to disrupt expression of the p65 subunit of NF-kappa B, all but two of the genes were shown to depend on this transcription factor for their expression, which correlated well with the existence of NF-kappa B binding sites in most of their promoters. Inflammatory, proapoptotic, and antiapoptotic genes were all shown to be regulated by NF-kappa B, demonstrating the wide variety of targets activated by NF-kappa B signaling and the necessity of differentiating among these genes for therapeutic purposes.
Similar articles
- Novel tumor necrosis factor alpha-regulated genes in rheumatoid arthritis.
Zhang HG, Hyde K, Page GP, Brand JP, Zhou J, Yu S, Allison DB, Hsu HC, Mountz JD. Zhang HG, et al. Arthritis Rheum. 2004 Feb;50(2):420-31. doi: 10.1002/art.20037. Arthritis Rheum. 2004. PMID: 14872484 - NF-kappaB-regulated expression of cellular FLIP protects rheumatoid arthritis synovial fibroblasts from tumor necrosis factor alpha-mediated apoptosis.
Bai S, Liu H, Chen KH, Eksarko P, Perlman H, Moore TL, Pope RM. Bai S, et al. Arthritis Rheum. 2004 Dec;50(12):3844-55. doi: 10.1002/art.20680. Arthritis Rheum. 2004. PMID: 15593196 - Regulation of monocyte chemoattractant protein-1 by the oxidized lipid, 13-hydroperoxyoctadecadienoic acid, in vascular smooth muscle cells via nuclear factor-kappa B (NF-kappa B).
Dwarakanath RS, Sahar S, Reddy MA, Castanotto D, Rossi JJ, Natarajan R. Dwarakanath RS, et al. J Mol Cell Cardiol. 2004 Apr;36(4):585-95. doi: 10.1016/j.yjmcc.2004.02.007. J Mol Cell Cardiol. 2004. PMID: 15081318 - [Alcohol action and activation of NF-kappa B].
Matsumoto H, Fukui Y. Matsumoto H, et al. Nihon Arukoru Yakubutsu Igakkai Zasshi. 2002 Apr;37(2):93-110. Nihon Arukoru Yakubutsu Igakkai Zasshi. 2002. PMID: 12043193 Review. Japanese. - [NF-kappa B and inflammatory intestinal diseases].
Nosti-Escanilla MP, Peña AS. Nosti-Escanilla MP, et al. Rev Esp Enferm Dig. 1998 Feb;90(2):113-9. Rev Esp Enferm Dig. 1998. PMID: 9580438 Review. Spanish.
Cited by
- Clonally expanded CD38hi cytotoxic CD8 T cells define the T cell infiltrate in checkpoint inhibitor-associated arthritis.
Wang R, Singaraju A, Marks KE, Shakib L, Dunlap G, Adejoorin I, Greisen SR, Chen L, Tirpack AK, Aude C, Fein MR, Todd DJ, MacFarlane L, Goodman SM, DiCarlo EF, Massarotti EM, Sparks JA, Jonsson AH, Brenner MB, Postow MA, Chan KK, Bass AR, Donlin LT, Rao DA. Wang R, et al. Sci Immunol. 2023 Jul 28;8(85):eadd1591. doi: 10.1126/sciimmunol.add1591. Epub 2023 Jul 28. Sci Immunol. 2023. PMID: 37506196 Free PMC article. - Significance of monocyte infiltration in patients with gastric cancer: A combined study based on single cell sequencing and TCGA.
Xu W, Zhao D, Huang X, Zhang M, Zhu W, Xu C. Xu W, et al. Front Oncol. 2022 Nov 21;12:1001307. doi: 10.3389/fonc.2022.1001307. eCollection 2022. Front Oncol. 2022. PMID: 36479092 Free PMC article. - Coilin as a regulator of NF-kB mediated inflammation in preeclampsia.
Logan MK, Lett KE, McLaurin DM, Hebert MD. Logan MK, et al. Biol Open. 2022 Jul 15;11(7):bio059326. doi: 10.1242/bio.059326. Epub 2022 Jul 25. Biol Open. 2022. PMID: 35762874 Free PMC article. - Selective pressure of endocrine therapy activates the integrated stress response through NFκB signaling in a subpopulation of ER positive breast cancer cells.
Semina SE, Pal P, Kansara NS, Huggins RJ, Alarid ET, Greene GL, Frasor J. Semina SE, et al. Breast Cancer Res. 2022 Mar 9;24(1):19. doi: 10.1186/s13058-022-01515-1. Breast Cancer Res. 2022. PMID: 35264224 Free PMC article. - A proteomic perspective on TNF-mediated signalling and cell death.
Tanzer MC. Tanzer MC. Biochem Soc Trans. 2022 Feb 28;50(1):13-20. doi: 10.1042/BST20211114. Biochem Soc Trans. 2022. PMID: 35166321 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources