Contribution of 5-hydroxytryptamine1B receptors and 20-hydroxyeiscosatetraenoic acid to fall in cerebral blood flow after subarachnoid hemorrhage - PubMed (original) (raw)
Comparative Study
Contribution of 5-hydroxytryptamine1B receptors and 20-hydroxyeiscosatetraenoic acid to fall in cerebral blood flow after subarachnoid hemorrhage
Liana Cambj-Sapunar et al. Stroke. 2003 May.
Abstract
Background and purpose: This study examined the interaction between 5-hydroxytryptamine1B (5-HT1B) receptors and 20-hydroxyeiscosatetraenoic acid (20-HETE) in contributing to the acute fall in regional cerebral blood flow (rCBF) after subarachnoid hemorrhage (SAH) in rats.
Methods: The effects of intracisternal injection of 0.3 mL of arterial blood, artificial cerebrospinal fluid, and 5-HT on rCBF and the levels of 20-HETE and 5-HT in cerebrospinal fluid were measured in rats pretreated with vehicle, a 5-HT1B receptor antagonist (isamoltane hemifumarate), or an inhibitor of the synthesis of 20-HETE (HET0016). The effects of HET0016 and isamoltane on the vasoconstrictor response and changes in [Ca2+]i to 5-HT were also studied in middle cerebral arteries and vascular smooth muscle cells isolated from these vessels.
Results: 20-HETE and 5-HT levels in cerebrospinal fluid rose from 172+/-10 to 629+/-44 ng/mL and from 6+/-4 to 1163+/-200 nmol/mL, respectively, after SAH. rCBF fell by 30% 10 minutes after SAH, and it remained at this level for the next 2 hours. Blockade of 5-HT1B receptors prevented the sustained fall in rCBF seen after SAH. Intracisternal injection of 5-HT mimicked SAH by increasing 20-HETE levels in cerebrospinal fluid to 475+/-94 ng/mL and reducing rCBF by 30%. Blockade of the synthesis of 20-HETE with HET0016 prevented the fall in rCBF produced by 5-HT. Isamoltane and HET0016 reduced the vasoconstrictor response of isolated MCA to 5-HT by >60% and diminished the rise in [Ca2+]i produced by 5-HT in vascular smooth muscle cells isolated from these arteries.
Conclusions: These results suggest that the release of 5-HT after SAH activates 5-HT1B receptors and the synthesis of 20-HETE and that 20-HETE contributes to the acute fall in rCBF by potentiating the vasoconstrictor response of cerebral vessels to 5-HT.
Similar articles
- 20-HETE contributes to the acute fall in cerebral blood flow after subarachnoid hemorrhage in the rat.
Kehl F, Cambj-Sapunar L, Maier KG, Miyata N, Kametani S, Okamoto H, Hudetz AG, Schulte ML, Zagorac D, Harder DR, Roman RJ. Kehl F, et al. Am J Physiol Heart Circ Physiol. 2002 Apr;282(4):H1556-65. doi: 10.1152/ajpheart.00924.2001. Am J Physiol Heart Circ Physiol. 2002. PMID: 11893593 - Effects of a 20-HETE antagonist and agonists on cerebral vascular tone.
Yu M, Cambj-Sapunar L, Kehl F, Maier KG, Takeuchi K, Miyata N, Ishimoto T, Reddy LM, Falck JR, Gebremedhin D, Harder DR, Roman RJ. Yu M, et al. Eur J Pharmacol. 2004 Feb 23;486(3):297-306. doi: 10.1016/j.ejphar.2004.01.009. Eur J Pharmacol. 2004. PMID: 14985052 - Reversal of delayed vasospasm by an inhibitor of the synthesis of 20-HETE.
Takeuchi K, Renic M, Bohman QC, Harder DR, Miyata N, Roman RJ. Takeuchi K, et al. Am J Physiol Heart Circ Physiol. 2005 Nov;289(5):H2203-11. doi: 10.1152/ajpheart.00556.2005. Epub 2005 Jun 17. Am J Physiol Heart Circ Physiol. 2005. PMID: 15964920 - Evidence that 20-HETE contributes to the development of acute and delayed cerebral vasospasm.
Roman RJ, Renic M, Dunn KM, Takeuchi K, Hacein-Bey L. Roman RJ, et al. Neurol Res. 2006 Oct;28(7):738-49. doi: 10.1179/016164106X152016. Neurol Res. 2006. PMID: 17164037 Review. - 5-HT receptors mediating external carotid vasoconstriction in vagosympathectomized dogs.
Villalón CM, Centurión D, Sánchez-López A, De Vries P, Saxena P. Villalón CM, et al. Zhongguo Yao Li Xue Bao. 1999 Dec;20(12):1057-67. Zhongguo Yao Li Xue Bao. 1999. PMID: 11216445 Review.
Cited by
- 20-Hydroxyeicosatetraenoic acid (20-HETE): Bioactions, receptors, vascular function, cardiometabolic disease and beyond.
Pascale JV, Wolf A, Kadish Y, Diegisser D, Kulaprathazhe MM, Yemane D, Ali S, Kim N, Baruch DE, Yahaya MAF, Dirice E, Adebesin AM, Falck JR, Schwartzman ML, Garcia V. Pascale JV, et al. Adv Pharmacol. 2023;97:229-255. doi: 10.1016/bs.apha.2023.01.002. Epub 2023 Feb 24. Adv Pharmacol. 2023. PMID: 37236760 Free PMC article. - The blood-brain barrier and the neurovascular unit in subarachnoid hemorrhage: molecular events and potential treatments.
Solár P, Zamani A, Lakatosová K, Joukal M. Solár P, et al. Fluids Barriers CNS. 2022 Apr 11;19(1):29. doi: 10.1186/s12987-022-00312-4. Fluids Barriers CNS. 2022. PMID: 35410231 Free PMC article. Review. - Vascular contributions to cognitive impairment and dementia: the emerging role of 20-HETE.
Gonzalez-Fernandez E, Liu Y, Auchus AP, Fan F, Roman RJ. Gonzalez-Fernandez E, et al. Clin Sci (Lond). 2021 Aug 13;135(15):1929-1944. doi: 10.1042/CS20201033. Clin Sci (Lond). 2021. PMID: 34374423 Free PMC article. Review. - Simultaneous Quantitation of Lipid Biomarkers for Inflammatory Bowel Disease Using LC-MS/MS.
Chhonker YS, Kanvinde S, Ahmad R, Singh AB, Oupický D, Murry DJ. Chhonker YS, et al. Metabolites. 2021 Feb 12;11(2):106. doi: 10.3390/metabo11020106. Metabolites. 2021. PMID: 33673198 Free PMC article. - 20-HETE Enzymes and Receptors in the Neurovascular Unit: Implications in Cerebrovascular Disease.
Gonzalez-Fernandez E, Staursky D, Lucas K, Nguyen BV, Li M, Liu Y, Washington C, Coolen LM, Fan F, Roman RJ. Gonzalez-Fernandez E, et al. Front Neurol. 2020 Sep 4;11:983. doi: 10.3389/fneur.2020.00983. eCollection 2020. Front Neurol. 2020. PMID: 33013649 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous