R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells - PubMed (original) (raw)
R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells
Kefei Yu et al. Nat Immunol. 2003 May.
Abstract
The mechanism responsible for immunoglobulin class switch recombination is unknown. Previous work has shown that class switch sequences have the unusual property of forming RNA-DNA hybrids when transcribed in vitro. Here we show that the RNA-DNA hybrid structure that forms in vitro is an R-loop with a displaced guanine (G)-rich strand that is single-stranded. This R-loop structure exists in vivo in B cells that have been stimulated to transcribe the gamma3 or the gamma2b switch region. The length of the R-loops can exceed 1 kilobase. We propose that this distinctive DNA structure is important in the class switch recombination mechanism
Comment in
- RNA AIDS DNA.
Fugmann SD, Schatz DG. Fugmann SD, et al. Nat Immunol. 2003 May;4(5):429-30. doi: 10.1038/ni0503-429. Nat Immunol. 2003. PMID: 12719733 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources