Differential gene expression in the rat caudate putamen after "binge" cocaine administration: advantage of triplicate microarray analysis - PubMed (original) (raw)
. 2003 Jun 15;48(4):157-69.
doi: 10.1002/syn.10198.
Affiliations
- PMID: 12687634
- DOI: 10.1002/syn.10198
Differential gene expression in the rat caudate putamen after "binge" cocaine administration: advantage of triplicate microarray analysis
Vadim Yuferov et al. Synapse. 2003.
Abstract
Rat genome U34A (Affymetrix) oligonucleotide microarrays were used to analyze changes in gene expression in the caudate putamen (CPu) of Fischer rats induced by 1 and 3 days of "binge" cocaine (or saline) administration. A triplicate array assay of pooled RNA of each treatment group was used to evaluate the technical variability and sensitivity of microarrays. Cocaine-regulated genes were identified using the Affymetrix MAS 5.0 and Data Mining Tool v. 3. Eighty-nine upregulated and eight downregulated genes/ESTs were found after 1 day of "binge" cocaine. Following 3 days of cocaine treatment we identified 21 upregulated and 17 downregulated genes/ESTs. RNase protection assays of selected genes confirmed reliability of changes identified by the microarrays at the level of > or =1.40-fold increase. Many genes upregulated in the CPu by cocaine were immediate early genes for transcription factors and for "effector" proteins (e.g., vesl/Homer1a, Arc, synaptotagmin IV). Acute "binge" cocaine also increased mRNA levels for glutamate receptor GluR2, dopamine receptor D1, and a number of phosphatases. Genes downregulated by cocaine include several genes associated with energy metabolism in mitochondria, as well as the phosphatydylinositol-4 kinase and the regulator of G-protein signaling protein 4 (RGS4). A differential expression of somatostatin receptor SSTR2, not known to be a cocaine-responsive gene, as well as the clock gene Per2, were found by microarrays and confirmed by RNase protection assay. These results demonstrate the potential of microarrays in profiling gene expression with > or =40% increase or > or =14% decrease in mRNA levels for discovery of novel cocaine-responsive genes.
Copyright 2003 Wiley-Liss, Inc.
Similar articles
- Regulation of kappa opioid receptor mRNA in the rat brain by "binge' pattern cocaine administration and correlation with preprodynorphin mRNA.
Spangler R, Ho A, Zhou Y, Maggos CE, Yuferov V, Kreek MJ. Spangler R, et al. Brain Res Mol Brain Res. 1996 May;38(1):71-6. doi: 10.1016/0169-328x(95)00319-n. Brain Res Mol Brain Res. 1996. PMID: 8737669 - Cocaine alters mu but not delta or kappa opioid receptor-stimulated in situ [35S]GTPgammaS binding in rat brain.
Schroeder JA, Niculescu M, Unterwald EM. Schroeder JA, et al. Synapse. 2003 Jan;47(1):26-32. doi: 10.1002/syn.10148. Synapse. 2003. PMID: 12422370 - Sensitized increase of period gene expression in the mouse caudate/putamen caused by repeated injection of methamphetamine.
Nikaido T, Akiyama M, Moriya T, Shibata S. Nikaido T, et al. Mol Pharmacol. 2001 Apr;59(4):894-900. doi: 10.1124/mol.59.4.894. Mol Pharmacol. 2001. PMID: 11259635 - Post-transcriptional regulation of dopamine D1 receptor expression in caudate-putamen of cocaine-sensitized mice.
Tobón KE, Catuzzi JE, Cote SR, Sonaike A, Kuzhikandathil EV. Tobón KE, et al. Eur J Neurosci. 2015 Jul;42(2):1849-57. doi: 10.1111/ejn.12933. Epub 2015 May 18. Eur J Neurosci. 2015. PMID: 25900179 Free PMC article.
Cited by
- Vilazodone, a Selective Serotonin Reuptake Inhibitor with Diminished Impact on Methylphenidate-Induced Gene Regulation in the Striatum: Role of 5-HT1A Receptor.
Hrabak M, Moon C, Bolaños-Guzmán CA, Steiner H. Hrabak M, et al. Mol Neurobiol. 2024 Apr;61(4):1907-1919. doi: 10.1007/s12035-023-03688-y. Epub 2023 Oct 9. Mol Neurobiol. 2024. PMID: 37807008 Free PMC article. - Altered expression of somatostatin signaling molecules and clock genes in the hippocampus of subjects with substance use disorder.
Valeri J, O'Donovan SM, Wang W, Sinclair D, Bollavarapu R, Gisabella B, Platt D, Stockmeier C, Pantazopoulos H. Valeri J, et al. Front Neurosci. 2022 Sep 7;16:903941. doi: 10.3389/fnins.2022.903941. eCollection 2022. Front Neurosci. 2022. PMID: 36161151 Free PMC article. - Altered cardiac mitochondrial dynamics and biogenesis in rat after short-term cocaine administration.
Wen S, Unuma K, Funakoshi T, Aki T, Uemura K. Wen S, et al. Sci Rep. 2021 Dec 16;11(1):24129. doi: 10.1038/s41598-021-03631-y. Sci Rep. 2021. PMID: 34916603 Free PMC article. - Fluoxetine Potentiates Oral Methylphenidate-Induced Gene Regulation in the Rat Striatum.
Moon C, Marion M, Thanos PK, Steiner H. Moon C, et al. Mol Neurobiol. 2021 Oct;58(10):4856-4870. doi: 10.1007/s12035-021-02466-y. Epub 2021 Jul 2. Mol Neurobiol. 2021. PMID: 34213723 Free PMC article. - Drug-activated cells: From immediate early genes to neuronal ensembles in addiction.
Salery M, Godino A, Nestler EJ. Salery M, et al. Adv Pharmacol. 2021;90:173-216. doi: 10.1016/bs.apha.2020.09.006. Epub 2021 Feb 19. Adv Pharmacol. 2021. PMID: 33706932 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources