Enzymatic profiling system in a small-molecule microarray - PubMed (original) (raw)

. 2003 Apr 17;5(8):1257-60.

doi: 10.1021/ol034233h.

Affiliations

• PMID: 12688733
• DOI: 10.1021/ol034233h

Enzymatic profiling system in a small-molecule microarray

Qing Zhu et al. Org Lett. 2003.

Abstract

[reaction: see text] We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.

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