Annexin I is an endogenous ligand that mediates apoptotic cell engulfment - PubMed (original) (raw)
Annexin I is an endogenous ligand that mediates apoptotic cell engulfment
Swathi Arur et al. Dev Cell. 2003 Apr.
Free article
Abstract
Engulfment of apoptotic cells requires presentation of new cell surface ligands by the dying cells. Using a differential proteomics technology, we identify that annexin I is a caspase-dependent engulfment ligand; it is recruited from the cytosol and exported to the outer plasma membrane leaflet, colocalizes with phosphatidylserine, and is required for efficient clearance of apoptotic cells. Furthermore, phosphatidylserine receptor (PSR) clustering around apoptotic cells indicates a requirement for annexin I. In the nematode Caenorhabditis elegans, downregulation of the annexin homolog prevents efficient engulfment of pharyngeal cell corpses. These results provide novel mechanistic insights into how apoptotic cells are removed and may explain a pathogenic mechanism of chronic inflammatory diseases where annexin I autoantibodies have been described.
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- P01 HL070694-010004/HL/NHLBI NIH HHS/United States
- P01 HL070694-019003/HL/NHLBI NIH HHS/United States
- P01 HL070694-036744/HL/NHLBI NIH HHS/United States
- P01 HL70694/HL/NHLBI NIH HHS/United States
- P20 GM065764-010003/GM/NIGMS NIH HHS/United States
- R01 HL 67569/HL/NHLBI NIH HHS/United States
- R01 HL067569-01/HL/NHLBI NIH HHS/United States
- R01 HL067569-02/HL/NHLBI NIH HHS/United States
- R01 HL067569-03/HL/NHLBI NIH HHS/United States
- R01 HL067569-04/HL/NHLBI NIH HHS/United States
- RR13186/RR/NCRR NIH HHS/United States
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