Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triphosphate energy-transducing system of Escherichia coli - PubMed (original) (raw)
Identification of the dicyclohexylcarbodiimide-reactive protein component of the adenosine 5'-triphosphate energy-transducing system of Escherichia coli
R H Fillingame. J Bacteriol. 1975 Nov.
Abstract
Membranes of Escherichia coli contain an adenosine 5'-triphosphate (ATP) energy-transducing system that is inhibited by treatment with dicyclohexylcarbodiimide (DCCD). The carbodiimide-reactive protein component of this system has been identified after treatment with [14C]DCCD. This protein has an apparent molecular weight of 9,000 as judged from acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and is extracted from the membrane with chloroform-methanol (2:1). These properties are similar to the analogous protein previously identified in mitochondria (Cattell et al., 1971). A mutant strain, RF-7, has been isolated which derives energy from oxidative phosphorylation in the presence of 5 mM DCCD. The ATP hydrolase activity of the membraned system in the mutant was considerably less sensitive to inhibition by DCCD than that in the wild type. The carbodiimide-reactive protein, which was easily labeled by [14C]DCCD in the wild type, was labeled much less rapidly in the carbodiimide-resistant mutant. It is thus concluded that the reaction of DCCD with this specific protein leads to inhibition of the ATP energy-transducing reactions. The mutation causing carbodiimide resistance in strain RF-7 was mapped. It is cotransduced with the uncA gene at a frequency exceeding 90%. The mutationally altered protein causing the carbodiimide resistance was not conclusively identified. However, reconstitution experiments indicate that the altered protein is not one of the subunits of the soluble ATP hydrolase activity, which can be removed from the membrane by washing with 1 mM tris(hydroxymethyl)aminomethane buffer lacking Mg2+. The carbodiimide-reactive protein remains with the membrane residue after removal of the soluble ATP hydrolase and is thus distinct from these subunits as well.
Similar articles
- Carbodiimide-resistant mutant of Escherichia coli: suppression of resistance to dicyclohexylcarbodiimide by growth on glucose or glycerol.
Fillingame RH, Wopat AE. Fillingame RH, et al. J Bacteriol. 1978 May;134(2):687-9. doi: 10.1128/jb.134.2.687-689.1978. J Bacteriol. 1978. PMID: 149107 Free PMC article. - Purification and characterization of the membrane adenosine triphosphatase complex from the wild-type and N,N'-dicyclohexylcarbodiimide-resistant strains of Streptococcus faecalis.
Leimgruber RM, Jensen C, Abrams A. Leimgruber RM, et al. J Bacteriol. 1981 Aug;147(2):363-72. doi: 10.1128/jb.147.2.363-372.1981. J Bacteriol. 1981. PMID: 6455413 Free PMC article.
Cited by
- Quinoline Compounds Targeting the _c_-Ring of ATP Synthase Inhibit Drug-Resistant Pseudomonas aeruginosa.
Fraunfelter VM, Pugh BA, Williams APL, Ward KT, Jackson DO, Austin M, Ciprich JF, Dippy L, Dunford J, Edwards GN, Glass E, Handy KM, Kellogg CN, Llewellyn K, Nyberg KQ, Shepard SJ, Thomas C, Wolfe AL, Steed PR. Fraunfelter VM, et al. ACS Infect Dis. 2023 Dec 8;9(12):2448-2456. doi: 10.1021/acsinfecdis.3c00317. Epub 2023 Nov 3. ACS Infect Dis. 2023. PMID: 37922420 Free PMC article. - Mutational analysis of a conserved positive charge in the c-ring of E. coli ATP synthase.
Shrestha RK, Founds MW, Shepard SJ, Rothrock MM, Defnet AE, Steed PR. Shrestha RK, et al. Biochim Biophys Acta Bioenerg. 2023 Apr 1;1864(2):148962. doi: 10.1016/j.bbabio.2023.148962. Epub 2023 Feb 21. Biochim Biophys Acta Bioenerg. 2023. PMID: 36822493 Free PMC article. - Synthesis and Evaluation of Pseudomonas aeruginosa ATP Synthase Inhibitors.
Ciprich JF, Buckhalt AJE, Carroll LL, Chen D, DeFiglia SA, McConnell RS, Parmar DJ, Pistor OL, Rao AB, Rubin ML, Volk GE, Steed PR, Wolfe AL. Ciprich JF, et al. ACS Omega. 2022 Aug 4;7(32):28434-28444. doi: 10.1021/acsomega.2c03127. eCollection 2022 Aug 16. ACS Omega. 2022. PMID: 35990476 Free PMC article. - Multiple metabolic changes mediate the response of Caenorhabditis elegans to the complex I inhibitor rotenone.
Gonzalez-Hunt CP, Luz AL, Ryde IT, Turner EA, Ilkayeva OR, Bhatt DP, Hirschey MD, Meyer JN. Gonzalez-Hunt CP, et al. Toxicology. 2021 Jan 15;447:152630. doi: 10.1016/j.tox.2020.152630. Epub 2020 Nov 11. Toxicology. 2021. PMID: 33188857 Free PMC article. - The Rnf complex is a Na+ coupled respiratory enzyme in a fermenting bacterium, Thermotoga maritima.
Kuhns M, Trifunović D, Huber H, Müller V. Kuhns M, et al. Commun Biol. 2020 Aug 7;3(1):431. doi: 10.1038/s42003-020-01158-y. Commun Biol. 2020. PMID: 32770029 Free PMC article.
References
- J Bacteriol. 1974 Jul;119(1):129-37 - PubMed
- J Biol Chem. 1974 Jul 25;249(14):4587-93 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- FEBS Lett. 1972 May 1;22(2):197-199 - PubMed
- Biochim Biophys Acta. 1972 Nov 17;283(2):217-22 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources