Adsorption of autoantibodies in the presence of LISS to detect alloantibodies underlying warm autoantibodies - PubMed (original) (raw)

Adsorption of autoantibodies in the presence of LISS to detect alloantibodies underlying warm autoantibodies

Jacques Chiaroni et al. Transfusion. 2003 May.

Abstract

Background: The safe transfusion of patients with warm autoimmune hemolytic anemia requires an efficient and time-saving assay to detect alloantibodies underlying autoantibodies. Methods used include RBCs treated with ZZAP reagent, proteolytic enzyme, or untreated RBCs in the presence of PEG. We propose a method using LISS, which presents some advantages over previous methods.

Study design and methods: We evaluated the effectiveness of autoantibody adsorption with papain-treated and untreated RBCs in the presence of LISS for removal of autoantibodies, without affecting alloantibodies.

Results: One-hundred twenty sera containing autoantibodies were adsorbed with our routine method, which uses papain-treated allogeneic RBCs. Seven-hundred twenty adsorptions (mean, 6 per sample) and 21,600 minutes (mean, 180 min per sample) were required to remove autoantibodies. Fifty sera were adsorbed with our routine method that uses papain-treated allogeneic RBCs in the presence of LISS. The number of adsorptions and the completion time were, respectively, 144 (mean, 2.9 per sample) and 2,880 minutes (mean, 57.6 min per sample). Twenty sera were evaluated with untreated autologous RBCs, in the presence of LISS; 58 adsorptions (mean, 2.9) and 1,160 minutes (mean, 58 min per sample) were required. Three adsorptions with antigen-negative allogeneic RBCs were performed on 8 sera containing alloantibodies with weak reactivity (anti-K [1], anti-D [1], anti-Fya[2], anti-S [2], anti-E [1], and anti-Jka[1]). Alloantibodies were detected with the LISS procedure with papain-treated and untreated RBCs and the routine papain method. Alloantibodies with weak reactivity, tested against the same antibody-detection RBCs, remained unchanged following adsorption of 5 sera. An anti-S, with very weak reactivity, was no longer detected, and an anti-Jka became weaker (1+), regardless of the procedure used. One anti-D became weaker only with the LISS adsorption method.

Conclusion: Autoantibodies can be adsorbed more efficiently in the presence of LISS.

PubMed Disclaimer

Cited by

Genetic diversity on the Comoros Islands shows early seafaring as major determinant of human biocultural evolution in the Western Indian Ocean.
Msaidie S, Ducourneau A, Boetsch G, Longepied G, Papa K, Allibert C, Yahaya AA, Chiaroni J, Mitchell MJ. Msaidie S, et al. Eur J Hum Genet. 2011 Jan;19(1):89-94. doi: 10.1038/ejhg.2010.128. Epub 2010 Aug 11. Eur J Hum Genet. 2011. PMID: 20700146 Free PMC article.
Utility of adsorption techniques in serological evaluation of warm autoimmune haemolytic anaemia.
Das SS, Chaudhary R. Das SS, et al. Blood Transfus. 2009 Oct;7(4):300-4. doi: 10.2450/2009.0079-08. Blood Transfus. 2009. PMID: 20011641 Free PMC article.
Panagglutination on the indirect antiglobulin test... this is the challenge!
Kokoris SI, Kalantzis D, Moschandreou D, Papaioannou K, Grouzi E. Kokoris SI, et al. Asian J Transfus Sci. 2022 Jul-Dec;16(2):257-262. doi: 10.4103/ajts.ajts_133_20. Epub 2022 Sep 28. Asian J Transfus Sci. 2022. PMID: 36687552 Free PMC article.
The role of the direct antiglobulin test in pre-transfusion investigations and the approach to selecting blood for transfusion in autoimmune haemolytic anaemia: results of a regional survey.
Liumbruno GM, Tognaccini A, Bonini R, Curciarello G, Masini I, Ringressi A, Tornabene F, Vanacore R. Liumbruno GM, et al. Blood Transfus. 2008 Jul;6(3):156-62. doi: 10.2450/2008.0046-07. Blood Transfus. 2008. PMID: 18705240 Free PMC article.

Adsorption of autoantibodies in the presence of LISS to detect alloantibodies underlying warm autoantibodies - PubMed (original) (raw)