Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling - PubMed (original) (raw)

. 2003 Apr 25;300(5619):650-3.

doi: 10.1126/science.1080405.

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Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling

Zachary A Wood et al. Science. 2003.

Abstract

Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.

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