The principle of gating charge movement in a voltage-dependent K+ channel - PubMed (original) (raw)
. 2003 May 1;423(6935):42-8.
doi: 10.1038/nature01581.
Affiliations
- PMID: 12721619
- DOI: 10.1038/nature01581
The principle of gating charge movement in a voltage-dependent K+ channel
Youxing Jiang et al. Nature. 2003.
Abstract
The steep dependence of channel opening on membrane voltage allows voltage-dependent K+ channels to turn on almost like a switch. Opening is driven by the movement of gating charges that originate from arginine residues on helical S4 segments of the protein. Each S4 segment forms half of a 'voltage-sensor paddle' on the channel's outer perimeter. Here we show that the voltage-sensor paddles are positioned inside the membrane, near the intracellular surface, when the channel is closed, and that the paddles move a large distance across the membrane from inside to outside when the channel opens. KvAP channels were reconstituted into planar lipid membranes and studied using monoclonal Fab fragments, a voltage-sensor toxin, and avidin binding to tethered biotin. Our findings lead us to conclude that the voltage-sensor paddles operate somewhat like hydrophobic cations attached to levers, enabling the membrane electric field to open and close the pore.
Comment in
- Structural biology: Life's transistors.
Sigworth FJ. Sigworth FJ. Nature. 2003 May 1;423(6935):21-2. doi: 10.1038/423021a. Nature. 2003. PMID: 12721605 No abstract available. - The voltage sensor of ion channels revealed.
Gregerson KA. Gregerson KA. Trends Endocrinol Metab. 2003 Aug;14(6):251-2. doi: 10.1016/s1043-2760(03)00110-3. Trends Endocrinol Metab. 2003. PMID: 12890583 No abstract available.
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