Electrophysiological analysis of neuronal chemokine receptors - PubMed (original) (raw)
Electrophysiological analysis of neuronal chemokine receptors
Seog Bae Oh et al. Methods. 2003 Apr.
Abstract
Several studies have shown that neurons in the central and peripheral nervous systems express a variety of chemokine receptors (CKRs). Activation of these receptors can influence neuronal signaling by regulating synaptic transmission and neuronal excitability. This article presents electrophysiological methods that are currently used to study the normal and pathophysiological role for CKRs in the nervous system. Conventional electrophysiological methods such as patch-clamp recording of isolated neurons, brain slices, and heterologous expression systems are described. In addition, single-cell reverse transcription-polymerase chain reaction is discussed as a technique that can be used in conjunction with patch-clamp recording to further investigate the molecular basis of neuronal CKR activation.
Similar articles
- Constitutive expression of CCR2 chemokine receptor and inhibition by MCP-1/CCL2 of GABA-induced currents in spinal cord neurones.
Gosselin RD, Varela C, Banisadr G, Mechighel P, Rostene W, Kitabgi P, Melik-Parsadaniantz S. Gosselin RD, et al. J Neurochem. 2005 Nov;95(4):1023-34. doi: 10.1111/j.1471-4159.2005.03431.x. Epub 2005 Sep 7. J Neurochem. 2005. PMID: 16150057 - Single-cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity.
Toledo-Rodriguez M, Markram H. Toledo-Rodriguez M, et al. Methods Mol Biol. 2014;1183:143-58. doi: 10.1007/978-1-4939-1096-0_8. Methods Mol Biol. 2014. PMID: 25023306 - The chemokine CCL2 modulates Ca2+ dynamics and electrophysiological properties of cultured cerebellar Purkinje neurons.
van Gassen KL, Netzeband JG, de Graan PN, Gruol DL. van Gassen KL, et al. Eur J Neurosci. 2005 Jun;21(11):2949-57. doi: 10.1111/j.1460-9568.2005.04113.x. Eur J Neurosci. 2005. PMID: 15978006 - Genes and channels: patch/voltage-clamp analysis and single-cell RT-PCR.
Sucher NJ, Deitcher DL, Baro DJ, Warrick RM, Guenther E. Sucher NJ, et al. Cell Tissue Res. 2000 Dec;302(3):295-307. doi: 10.1007/s004410000289. Cell Tissue Res. 2000. PMID: 11151442 Review. - [Analysis of molecular basis of neuronal properties using the patch-clamp RT-PCR method].
Tsuzuki K. Tsuzuki K. Nihon Rinsho. 1998 Jul;56(7):1681-7. Nihon Rinsho. 1998. PMID: 9702037 Review. Japanese.
Cited by
- Neuronal chemokines: versatile messengers in central nervous system cell interaction.
de Haas AH, van Weering HR, de Jong EK, Boddeke HW, Biber KP. de Haas AH, et al. Mol Neurobiol. 2007 Oct;36(2):137-51. doi: 10.1007/s12035-007-0036-8. Epub 2007 Jul 10. Mol Neurobiol. 2007. PMID: 17952658 Free PMC article. Review. - A Potential Contribution of Chemokine Network Dysfunction to the Depressive Disorders.
Ślusarczyk J, Trojan E, Chwastek J, Głombik K, Basta-Kaim A. Ślusarczyk J, et al. Curr Neuropharmacol. 2016;14(7):705-20. doi: 10.2174/1570159x14666160219131357. Curr Neuropharmacol. 2016. PMID: 26893168 Free PMC article. Review. - The Role of Chemokines in the Pathophysiology of Major Depressive Disorder.
Milenkovic VM, Stanton EH, Nothdurfter C, Rupprecht R, Wetzel CH. Milenkovic VM, et al. Int J Mol Sci. 2019 May 9;20(9):2283. doi: 10.3390/ijms20092283. Int J Mol Sci. 2019. PMID: 31075818 Free PMC article. Review. - Fractalkine Attenuates Microglial Cell Activation Induced by Prenatal Stress.
Ślusarczyk J, Trojan E, Głombik K, Chamera K, Roman A, Budziszewska B, Basta-Kaim A. Ślusarczyk J, et al. Neural Plast. 2016;2016:7258201. doi: 10.1155/2016/7258201. Epub 2016 Apr 27. Neural Plast. 2016. PMID: 27239349 Free PMC article. Retracted. - Evidence for TNFα action on excitatory and inhibitory neurotransmission in the central amygdala: a brain site influenced by stress.
Ming Z, Criswell HE, Breese GR. Ming Z, et al. Brain Behav Immun. 2013 Oct;33:102-11. doi: 10.1016/j.bbi.2013.06.001. Epub 2013 Jun 11. Brain Behav Immun. 2013. PMID: 23770090 Free PMC article.