Modulation of ion conductance and active transport by TGF-beta 1 in alveolar epithelial cell monolayers - PubMed (original) (raw)
. 2003 Dec;285(6):L1192-200.
doi: 10.1152/ajplung.00379.2002. Epub 2003 May 2.
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- PMID: 12730080
- DOI: 10.1152/ajplung.00379.2002
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Modulation of ion conductance and active transport by TGF-beta 1 in alveolar epithelial cell monolayers
Brigham C Willis et al. Am J Physiol Lung Cell Mol Physiol. 2003 Dec.
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Abstract
Transforming growth factor-beta1 (TGF-beta 1) may be a critical mediator of lung injury and subsequent remodeling during recovery. We evaluated the effects of TGF-beta 1 on the permeability and active ion transport properties of alveolar epithelial cell monolayers. Rat alveolar type II cells plated on polycarbonate filters in defined serum-free medium form confluent monolayers and acquire the phenotypic characteristics of alveolar type I cells. Exposure to TGF-beta 1 (0.1-100 pM) from day 0 resulted in a concentration- and time-dependent decrease in transepithelial resistance (Rt) and increase in short-circuit current (Isc). Apical amiloride or basolateral ouabain on day 6 inhibited Isc by 80 and 100%, respectively. Concurrent increases in expression of Na+-K+-ATPase alpha 1- and beta 1-subunits were observed in TGF-beta 1-treated monolayers. No change in the alpha-subunit of the rat epithelial sodium channel (alpha-rENaC) was seen. Exposure of confluent monolayers to TGF-beta 1 from day 4 resulted in an initial decrease in Rt within 6 h, followed by an increase in Isc over 72-96 h. These results demonstrate that TGF-beta 1 modulates ion conductance and active transport characteristics of the alveolar epithelium, associated with increased Na+-K+-ATPase, but without a change in alpha-rENaC.
Comment in
- Transforming growth factor-beta 1 regulates lung epithelial barrier function and fluid transport.
Clerici C, Matthay MA. Clerici C, et al. Am J Physiol Lung Cell Mol Physiol. 2003 Dec;285(6):L1190-1. doi: 10.1152/ajplung.00230.2003. Am J Physiol Lung Cell Mol Physiol. 2003. PMID: 14604849 Review. No abstract available.
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