Inhaled NO inhibits platelet aggregation and elevates plasma but not intraplatelet cGMP in healthy human volunteers - PubMed (original) (raw)

Clinical Trial

. 2003 Aug;285(2):H637-42.

doi: 10.1152/ajpheart.00622.2002. Epub 2003 May 15.

Affiliations

• PMID: 12750066
• DOI: 10.1152/ajpheart.00622.2002

Free article

Clinical Trial

Inhaled NO inhibits platelet aggregation and elevates plasma but not intraplatelet cGMP in healthy human volunteers

Maurice Beghetti et al. Am J Physiol Heart Circ Physiol. 2003 Aug.

Free article

Abstract

Effects of inhaled nitric oxide (NO) on human platelet function are controversial. It is uncertain whether intraplatelet cGMP mediates the effect of inhaled NO on platelet function. We investigated the effect of 30 ppm inhaled NO on platelet aggregation and plasma and intraplatelet cGMP in 12 subjects. We performed platelet aggregation studies by using a photooptical aggregometer and five agonists (ADP, collagen, epinephrine, arachidonic acid, and ristocetin). During inhalation, the maximal extent of platelet aggregation decreased by 75% with epinephrine (P < 0.005), 56% with collagen (P < 0.005), and 20% with arachidonic acid (P < 0.05). Responses to ADP (8% P > 0.05) and ristocetin (5% P > 0.05) were unaffected. Platelet aggregation velocity decreased by 64% with collagen (P < 0.005), 60% with epinephrine (P < 0.05), 33% with arachidonic acid (P < 0.05), and 14% with ADP (P > 0.05). Plasma cGMP levels increased from 2.58 +/- 0.43 to 9.99 +/- 5.57 pmol/ml (P < 0.005), intraplatelet cGMP levels were unchanged (means +/- SD: 1.96 +/- 0.58 vs. 2.71 +/- 1.67 pmol/109 platelets; P > 0.05). Inhaled NO inhibits platelet aggregation via a cGMP independent mechanism.

PubMed Disclaimer

Similar articles

• Inhaled nitric oxide inhibits human platelet aggregation, P-selectin expression, and fibrinogen binding in vitro and in vivo.
  Gries A, Bode C, Peter K, Herr A, Böhrer H, Motsch J, Martin E. Gries A, et al. Circulation. 1998 Apr 21;97(15):1481-7. doi: 10.1161/01.cir.97.15.1481. Circulation. 1998. PMID: 9576429 Clinical Trial.
• Nitric oxide inhalation inhibits platelet aggregation and platelet-mediated pulmonary thrombosis in rats.
  Nong Z, Hoylaerts M, Van Pelt N, Collen D, Janssens S. Nong Z, et al. Circ Res. 1997 Nov;81(5):865-9. doi: 10.1161/01.res.81.5.865. Circ Res. 1997. PMID: 9351461
• Suppressed anti-aggregating and cGMP-elevating effects of sodium nitroprusside in platelets from patients with stable angina pectoris.
  Chirkov YY, Chirkova LP, Horowitz JD. Chirkov YY, et al. Naunyn Schmiedebergs Arch Pharmacol. 1996 Oct;354(4):520-5. doi: 10.1007/BF00168445. Naunyn Schmiedebergs Arch Pharmacol. 1996. PMID: 8897457
• Organic nitrates and compounds that increase intraplatelet cyclic guanosine monophosphate (cGMP) levels enhance the antiaggregating effects of the stable prostacyclin analogue iloprost.
  Anfossi G, Massucco P, Mularoni E, Cavalot F, Mattiello L, Trovati M. Anfossi G, et al. Prostaglandins Leukot Essent Fatty Acids. 1993 Nov;49(5):839-45. doi: 10.1016/0952-3278(93)90207-d. Prostaglandins Leukot Essent Fatty Acids. 1993. PMID: 7508131
• Adenosine increases human platelet levels of cGMP through nitric oxide: possible role in its antiaggregating effect.
  Anfossi G, Russo I, Massucco P, Mattiello L, Cavalot F, Balbo A, Trovati M. Anfossi G, et al. Thromb Res. 2002 Jan 1;105(1):71-8. doi: 10.1016/s0049-3848(01)00412-1. Thromb Res. 2002. PMID: 11864710

Cited by

• Effects of inhaled nitric oxide on hemostasis in healthy adults treated with heparin: a randomized, controlled, blinded crossover study.
  Goldstein B, Baldassarre J, Young JN. Goldstein B, et al. Thromb J. 2012 Jan 9;10:1. doi: 10.1186/1477-9560-10-1. Thromb J. 2012. PMID: 22229969 Free PMC article.
• S-nitrosylation in cardiovascular signaling.
  Lima B, Forrester MT, Hess DT, Stamler JS. Lima B, et al. Circ Res. 2010 Mar 5;106(4):633-46. doi: 10.1161/CIRCRESAHA.109.207381. Circ Res. 2010. PMID: 20203313 Free PMC article. Review.
• Platelets in pulmonary hypertension: a causative role or a simple association?
  Zanjani KS. Zanjani KS. Iran J Pediatr. 2012 Jun;22(2):145-57. Iran J Pediatr. 2012. PMID: 23056879 Free PMC article.
• Nitric oxide regulation of protein trafficking in the cardiovascular system.
  Lowenstein CJ. Lowenstein CJ. Cardiovasc Res. 2007 Jul 15;75(2):240-6. doi: 10.1016/j.cardiores.2007.03.024. Epub 2007 Apr 3. Cardiovasc Res. 2007. PMID: 17490627 Free PMC article. Review.
• Regulation of platelet granule exocytosis by S-nitrosylation.
  Morrell CN, Matsushita K, Chiles K, Scharpf RB, Yamakuchi M, Mason RJ, Bergmeier W, Mankowski JL, Baldwin WM 3rd, Faraday N, Lowenstein CJ. Morrell CN, et al. Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3782-7. doi: 10.1073/pnas.0408310102. Epub 2005 Feb 28. Proc Natl Acad Sci U S A. 2005. PMID: 15738422 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon