The FimH A27V mutation is pathoadaptive for urovirulence in Escherichia coli B2 phylogenetic group isolates - PubMed (original) (raw)

The FimH A27V mutation is pathoadaptive for urovirulence in Escherichia coli B2 phylogenetic group isolates

Florence Hommais et al. Infect Immun. 2003 Jun.

Abstract

Correlations between FimH mutations and virulence were established by studying a collection of human commensal and extraintestinal pathogenic Escherichia coli natural isolates. Pathoadaptive (A27V and, to a lesser extent, A119V) and "commensal-adaptive" (A202V) mutations were evidenced in B2 phylogenetic group strains. fimH phylogenetic analysis indicates that these pathoadaptive mutations occurred several times.

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Figures

FIG. 1.

FIG. 1.

Unrooted semistrict consensus tree reconstructed from the fimH gene sequences by using parsimony in PAUP* (20); 696 nucleotides, of which 62 are informative for parsimony (l = 241; consistency index, 0.456; retention index, 0.877). The numbers at branches are the bootstrap proportions obtained from 100 replicates. Only bootstraps with proportions above 50% are given. The phylogenetic groups to which the strains belong, determined as described in reference , are indicated: A, B1, D, and B2. Similar topology was obtained with a neighbor-joining approach (data not shown). Strains within circles correspond to the strains with the A27V, N70S/S78N, A119V, and A202V mutations. K12 and CFT073 sequences have been extracted from GenBank.

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