Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS) - PubMed (original) (raw)

Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS)

Leo L M Poon et al. Clin Chem. 2003 Jun.

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Figure 1.

Figure 1.

Detection of a novel human coronavirus associated with SARS by real-time quantitative PCR (a), and melting curve analysis of PCR products from clinical samples (b). (a), amplification plot of fluorescence intensity against the PCR cycle. The copy numbers of input plasmid DNA in the reactions are indicated. The x axis denotes the cycle number of a quantitative PCR assay, and the y axis denotes the fluorescence intensity (F1) over the background. (b), melting curve analysis of PCR products from clinical samples. Signals from positive (+ve) samples, negative (−ve) samples, and a water control (water) are indicated. The x axis denotes the temperature (°C), and the y axis denotes the fluorescence intensity (F1) over the background.

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References

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