hFis1, a novel component of the mammalian mitochondrial fission machinery - PubMed (original) (raw)

. 2003 Sep 19;278(38):36373-9.

doi: 10.1074/jbc.M303758200. Epub 2003 Jun 3.

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• PMID: 12783892
• DOI: 10.1074/jbc.M303758200

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hFis1, a novel component of the mammalian mitochondrial fission machinery

Dominic I James et al. J Biol Chem. 2003.

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Erratum in

• J Biol Chem. 2004 Aug 20;279(34):36166

Abstract

The balance between the fission and fusion mechanisms regulate the morphology of mitochondria. In this study we have identified a mammalian protein that we call hFis1, which is the orthologue of the yeast Fis1p known to participate in yeast mitochondrial division. hFis1, when overexpressed in various cell types, localized to the outer mitochondrial membrane and induced mitochondrial fission. This event was inhibited by a dominant negative mutant of Drp1 (Drp1(K38A)), a major component of the fission apparatus. Fragmentation of the mitochondrial network by hFis1 was followed by the release of cytochrome c and ultimately apoptosis. Bcl-xL was able to block cytochrome c release and apoptosis but failed to prevent mitochondrial fragmentation. Our studies show that hFis1 is part of the mammalian fission machinery and suggest that regulation of the fission processes might be involved in apoptotic mechanisms.

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