Three modes of synaptic vesicular recycling revealed by single-vesicle imaging - PubMed (original) (raw)
. 2003 Jun 5;423(6940):607-13.
doi: 10.1038/nature01677.
Affiliations
- PMID: 12789331
- DOI: 10.1038/nature01677
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
Sunil P Gandhi et al. Nature. 2003.
Abstract
Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a slow (8-21 s) 'compensatory' mode; and a 'stranded' mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.
Comment in
- Neurobiology: All change at the synapse.
Rizzoli SO, Betz WJ. Rizzoli SO, et al. Nature. 2003 Jun 5;423(6940):591-2. doi: 10.1038/423591a. Nature. 2003. PMID: 12789318 No abstract available.
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