Bovine non-O157 Shiga toxin 2-containing Escherichia coli isolates commonly possess stx2-EDL933 and/or stx2vhb subtypes - PubMed (original) (raw)

Bovine non-O157 Shiga toxin 2-containing Escherichia coli isolates commonly possess stx2-EDL933 and/or stx2vhb subtypes

Kim N Brett et al. J Clin Microbiol. 2003 Jun.

Abstract

stx(2) genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx(2-EDL933) (103 of 127; 81.1%) either in combination with stx(2vhb) (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx(2-EDL933) and stx(2vhb); 2 isolates contained stx(2vhb) only. Although 7 of 11 (63.6%) human O113:H21 isolates associated with diarrhea possessed stx(2-EDL933), the remaining 4 isolates possessed a combination of stx(2-EDL933) and stx(2vhb). Three of the four were from separate sporadic cases of HUS, and one was from an unknown source.

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Figures

FIG. 1.

FIG. 1.

_Hae_III (A) and _Pvu_II (B) digests of PCR products obtained with VT2-e and VT2-f primers and _Hin_cII (C) digests of PCR products obtained with Lin F and Lin R primers. Lanes: 1, 100-bp Plus marker; 2, _stx_2d-Ount variant of serotype O91:H−; 3, _stx_2-EDL933/_stx_2vhb variant of serotype O113:H21; 4, _stx_2-EDL933/_stx_2vhb variant of serotype O113:H21; 5, _stx_2-EDL933/_stx_2vhb variant of serotype O113:H21; 6, _stx_2-EDL933/_stx_2vhb variant of serotype O110:H40; 7, _stx_2-EDL933 variant of serotype Ont:H5; 8, _stx_2vhb variant of serotype Ont:HR; 9, _stx_2-OX3a variant of serotype O5:H−; 10, _stx_2vhb variant of serotype Ont:H11.

FIG. 2.

FIG. 2.

_Msp_I (A), _Nci_I (B), and _Rsa_I (C) digests of PCR products obtained with Tyler F and Tyler R primers. Lanes: 1, 100-bp Plus marker; 2, _stx_2-EDL933/_stx_2vhb variant of serotype O113:H21; 3, _stx_2vhb variant of serotype Ont:HR; 4, _stx_2-EDL933 variant of serotype Ont:H5; 5, _stx_2-EDL933 variant of serotype Ont:H5.

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