Application of a novel immunomagnetic separation-bacteriophage assay for the detection of Salmonella enteritidis and Escherichia coli O157:H7 in food - PubMed (original) (raw)
Application of a novel immunomagnetic separation-bacteriophage assay for the detection of Salmonella enteritidis and Escherichia coli O157:H7 in food
Stacy J Favrin et al. Int J Food Microbiol. 2003.
Abstract
Salmonella infection is the second most prevalent cause of foodborne illness in most developing countries. Meat, poultry, and dairy products are frequently implicated in outbreaks. The objective of this study was to apply a novel immunomagnetic separation (IMS)-bacteriophage assay to the detection of Salmonella enteritidis in artificially inoculated skimmed milk powder, chicken rinses, and ground beef. In all food types tested, the IMS-bacteriophage assay was able to detect an average of 3 CFU of S. enteritidis in 25 g or ml of food sample. Total assay time including pre-enrichment is about 20 h. The results indicate that the IMS-bacteriophage assay is a rapid and sensitive means of detecting S. enteritidis in these foods. The assay was successfully adapted to the detection of Escherichia coli O157:H7 and was able to detect E. coli in ground beef at the lowest inoculation level tested, 2 CFU/g. The assay was also adapted to the simultaneous detection of S. enteritidis and E. coli. The results indicate that the IMS-bacteriophage assay shows promise for the simultaneous detection of these pathogens, but further development work would be necessary to improve sensitivity and produce reliable results at low inoculation levels.
Similar articles
- Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification.
Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S. Aydin M, et al. J Food Prot. 2014 Jan;77(1):100-5. doi: 10.4315/0362-028X.JFP-13-274. J Food Prot. 2014. PMID: 24406005 - The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk.
Goodridge L, Chen J, Griffiths M. Goodridge L, et al. Int J Food Microbiol. 1999 Mar 1;47(1-2):43-50. doi: 10.1016/s0168-1605(99)00010-0. Int J Food Microbiol. 1999. PMID: 10357272 - Detection of E. coli O157:H7 in raw ground beef by Pathatrix™ immunomagnetic-separation, real-time PCR and cultural methods.
Fedio WM, Jinneman KC, Yoshitomi KJ, Zapata R, Wendakoon CN, Browning P, Weagant SD. Fedio WM, et al. Int J Food Microbiol. 2011 Aug 2;148(2):87-92. doi: 10.1016/j.ijfoodmicro.2011.05.005. Epub 2011 May 13. Int J Food Microbiol. 2011. PMID: 21641670 - Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection.
Wang Z, Cai R, Gao Z, Yuan Y, Yue T. Wang Z, et al. Compr Rev Food Sci Food Saf. 2020 Nov;19(6):3802-3824. doi: 10.1111/1541-4337.12656. Epub 2020 Oct 18. Compr Rev Food Sci Food Saf. 2020. PMID: 33337037 Review. - Recent pre-harvest supplementation strategies to reduce carriage and shedding of zoonotic enteric bacterial pathogens in food animals.
Callaway TR, Anderson RC, Edrington TS, Genovese KJ, Harvey RB, Poole TL, Nisbet DJ. Callaway TR, et al. Anim Health Res Rev. 2004 Jun;5(1):35-47. doi: 10.1079/ahr200462. Anim Health Res Rev. 2004. PMID: 15460539 Review.
Cited by
- Novel phage-based bio-processing of pathogenic Escherichia coli and its biofilms.
Jassim SA, Abdulamir AS, Abu Bakar F. Jassim SA, et al. World J Microbiol Biotechnol. 2012 Jan;28(1):47-60. doi: 10.1007/s11274-011-0791-6. Epub 2011 May 22. World J Microbiol Biotechnol. 2012. PMID: 22806779 - In vitro Evaluation of the Antiviral Activity of an Extract of Date Palm (Phoenix dactylifera L.) Pits on a Pseudomonas Phage.
Jassim SA, Naji MA. Jassim SA, et al. Evid Based Complement Alternat Med. 2010 Mar;7(1):57-62. doi: 10.1093/ecam/nem160. Epub 2007 Oct 27. Evid Based Complement Alternat Med. 2010. PMID: 18955267 Free PMC article. - Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids.
Chang SC, Anderson TI, Bahrman SE, Gruden CL, Khijniak AI, Adriaens P. Chang SC, et al. J Ind Microbiol Biotechnol. 2005 Dec;32(11-12):629-38. doi: 10.1007/s10295-005-0238-x. Epub 2005 Jun 3. J Ind Microbiol Biotechnol. 2005. PMID: 15933873 - Accurate and sensitive detection of Salmonella in foods by engineered bacteriophages.
Nguyen MM, Gil J, Brown M, Cesar Tondo E, Soraya Martins de Aquino N, Eisenberg M, Erickson S. Nguyen MM, et al. Sci Rep. 2020 Oct 15;10(1):17463. doi: 10.1038/s41598-020-74587-8. Sci Rep. 2020. PMID: 33060781 Free PMC article. - Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells.
Foddai A, Elliott CT, Grant IR. Foddai A, et al. Appl Environ Microbiol. 2010 Nov;76(22):7550-8. doi: 10.1128/AEM.01432-10. Epub 2010 Sep 17. Appl Environ Microbiol. 2010. PMID: 20851966 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources