Spatiotemporal calcium signaling in a Drosophila melanogaster cell line stably expressing a Drosophila muscarinic acetylcholine receptor - PubMed (original) (raw)

Spatiotemporal calcium signaling in a Drosophila melanogaster cell line stably expressing a Drosophila muscarinic acetylcholine receptor

D Cordova et al. Invert Neurosci. 2003 Nov.

Abstract

A muscarinic acetylcholine receptor (mAChR), DM1, expressed in the nervous system of Drosophila melanogaster, has been stably expressed in a Drosophila S2 cell line (S2-DM1) and used to investigate spatiotemporal calcium changes following agonist activation. Carbamylcholine (CCh) and oxotremorine are potent agonists, whereas application of the vertebrate M1 mAChR agonist, McN-A-343, results in a weak response. Activation of S2-DM1 receptors using CCh resulted in an increase in intracellular calcium ([Ca(2+)](i)) that was biphasic. Two distinct calcium sources were found to contribute to calcium signaling: (1) internal stores that are sensitive to both thapsigargin and 2-aminoethoxydiphenyl borate and (2) capacitative calcium entry. Spatiotemporal imaging of individual S2-DM1 cells showed that the CCh-induced [Ca(2+)](i) transient resulted from a homogeneous calcium increase throughout the cell, indicative of calcium release from internal stores. In contrast, ionomycin induced the formation of a "calcium ring" at the cell periphery, consistent with external calcium influx.

PubMed Disclaimer

Cited by

Candidate neural substrates for off-edge motion detection in Drosophila.
Shinomiya K, Karuppudurai T, Lin TY, Lu Z, Lee CH, Meinertzhagen IA. Shinomiya K, et al. Curr Biol. 2014 May 19;24(10):1062-70. doi: 10.1016/j.cub.2014.03.051. Epub 2014 Apr 24. Curr Biol. 2014. PMID: 24768048 Free PMC article.
A genetic RNAi screen for IP₃/Ca²⁺ coupled GPCRs in Drosophila identifies the PdfR as a regulator of insect flight.
Agrawal T, Sadaf S, Hasan G. Agrawal T, et al. PLoS Genet. 2013;9(10):e1003849. doi: 10.1371/journal.pgen.1003849. Epub 2013 Oct 3. PLoS Genet. 2013. PMID: 24098151 Free PMC article.
Molecular basis of the CRAC channel.
Cahalan MD, Zhang SL, Yeromin AV, Ohlsen K, Roos J, Stauderman KA. Cahalan MD, et al. Cell Calcium. 2007 Aug;42(2):133-44. doi: 10.1016/j.ceca.2007.03.002. Epub 2007 May 7. Cell Calcium. 2007. PMID: 17482674 Free PMC article. Review.
A store-operated Ca(2+) influx pathway in the bag cell neurons of Aplysia.
Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, Magoski NS. Kachoei BA, et al. J Neurophysiol. 2006 Nov;96(5):2688-98. doi: 10.1152/jn.00118.2006. Epub 2006 Aug 2. J Neurophysiol. 2006. PMID: 16885525 Free PMC article.
A store-operated calcium channel in Drosophila S2 cells.
Yeromin AV, Roos J, Stauderman KA, Cahalan MD. Yeromin AV, et al. J Gen Physiol. 2004 Feb;123(2):167-82. doi: 10.1085/jgp.200308982. J Gen Physiol. 2004. PMID: 14744989 Free PMC article.

References

1. J Neurophysiol. 1994 Oct;72(4):1862-73 - PubMed
1. Biochem J. 2001 Oct 15;359(Pt 2):435-41 - PubMed
1. Cell Calcium. 1998 Apr;23(4):219-28 - PubMed
1. Cell. 1996 May 31;85(5):661-71 - PubMed
1. Cell Calcium. 1990 Nov-Dec;11(10):611-24 - PubMed

Spatiotemporal calcium signaling in a Drosophila melanogaster cell line stably expressing a Drosophila muscarinic acetylcholine receptor - PubMed (original) (raw)