Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii - PubMed (original) (raw)

Comparative Study

Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii

Lan Huang et al. J Struct Funct Genomics. 2002.

Abstract

We have determined the three-dimensional (3-D) structure of protein MJ0882, which derives from a hypothetical open reading frame in the genome of the hyperthermophile Methanococcus jannaschii. The 3-D fold of MJ0882 at 1.8 A highly resembles that of a methyltransferase, despite limited sequence similarity to any confirmed methyltransferase. The structure has an S-adenosylmethionine (AdoMet) binding pocket surrounded by motifs with similarities to those commonly found among AdoMet binding proteins. Preliminary biochemical experiments show that MJ0882 specifically binds to AdoMet, which is the essential co-factor for methyltransferases.

PubMed Disclaimer

Similar articles

• Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes.
  Huang Y, Komoto J, Konishi K, Takata Y, Ogawa H, Gomi T, Fujioka M, Takusagawa F. Huang Y, et al. J Mol Biol. 2000 Apr 21;298(1):149-62. doi: 10.1006/jmbi.2000.3637. J Mol Biol. 2000. PMID: 10756111
• Structure-based sequence alignment of three AdoMet-dependent DNA methyltransferases.
  O'Gara M, McCloy K, Malone T, Cheng X. O'Gara M, et al. Gene. 1995 May 19;157(1-2):135-8. doi: 10.1016/0378-1119(94)00669-j. Gene. 1995. PMID: 7607477
• Functional roles of conserved amino acid residues in DNA methyltransferases investigated by site-directed mutagenesis of the EcoRV adenine-N6-methyltransferase.
  Roth M, Helm-Kruse S, Friedrich T, Jeltsch A. Roth M, et al. J Biol Chem. 1998 Jul 10;273(28):17333-42. doi: 10.1074/jbc.273.28.17333. J Biol Chem. 1998. PMID: 9651316
• Structure, function and physiological role of glycine N-methyltransferase.
  Ogawa H, Gomi T, Takusagawa F, Fujioka M. Ogawa H, et al. Int J Biochem Cell Biol. 1998 Jan;30(1):13-26. doi: 10.1016/s1357-2725(97)00105-2. Int J Biochem Cell Biol. 1998. PMID: 9597750 Review.
• Structure and function of DNA methyltransferases.
  Cheng X. Cheng X. Annu Rev Biophys Biomol Struct. 1995;24:293-318. doi: 10.1146/annurev.bb.24.060195.001453. Annu Rev Biophys Biomol Struct. 1995. PMID: 7663118 Review.

Cited by

• Protein function annotation by local binding site surface similarity.
  Spitzer R, Cleves AE, Varela R, Jain AN. Spitzer R, et al. Proteins. 2014 Apr;82(4):679-94. doi: 10.1002/prot.24450. Epub 2013 Nov 22. Proteins. 2014. PMID: 24166661 Free PMC article.
• Structural and functional insights into the molecular mechanism of rRNA m6A methyltransferase RlmJ.
  Punekar AS, Liljeruhm J, Shepherd TR, Forster AC, Selmer M. Punekar AS, et al. Nucleic Acids Res. 2013 Nov;41(20):9537-48. doi: 10.1093/nar/gkt719. Epub 2013 Aug 13. Nucleic Acids Res. 2013. PMID: 23945937 Free PMC article.
• Structure-based inference of molecular functions of proteins of unknown function from Berkeley Structural Genomics Center.
  Shin DH, Hou J, Chandonia JM, Das D, Choi IG, Kim R, Kim SH. Shin DH, et al. J Struct Funct Genomics. 2007 Sep;8(2-3):99-105. doi: 10.1007/s10969-007-9025-4. Epub 2007 Sep 2. J Struct Funct Genomics. 2007. PMID: 17764033 Review.
• Ribosomal RNA guanine-(N2)-methyltransferases and their targets.
  Sergiev PV, Bogdanov AA, Dontsova OA. Sergiev PV, et al. Nucleic Acids Res. 2007;35(7):2295-301. doi: 10.1093/nar/gkm104. Epub 2007 Mar 27. Nucleic Acids Res. 2007. PMID: 17389639 Free PMC article. Review.
• Biochemical and genetic analysis of RNA cap guanine-N2 methyltransferases from Giardia lamblia and Schizosaccharomyces pombe.
  Hausmann S, Ramirez A, Schneider S, Schwer B, Shuman S. Hausmann S, et al. Nucleic Acids Res. 2007;35(5):1411-20. doi: 10.1093/nar/gkl1150. Epub 2007 Feb 6. Nucleic Acids Res. 2007. PMID: 17284461 Free PMC article.

References

1. 1. Biochemistry. 1998 May 19;37(20):7103-12 - PubMed
2. 1. Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):487-93 - PubMed
3. 1. J Mol Biol. 1991 Sep 5;221(1):31-4 - PubMed
4. 1. Methods Enzymol. 1997;276:530-7 - PubMed
5. 1. Cell. 1995 Jul 14;82(1):143-53 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Ovid Technologies, Inc.

• Research Materials

  • Addgene Non-profit plasmid repository