Continuous T cell receptor signaling required for synapse maintenance and full effector potential - PubMed (original) (raw)
doi: 10.1038/ni951. Epub 2003 Jul 13.
Affiliations
- PMID: 12858171
- DOI: 10.1038/ni951
Continuous T cell receptor signaling required for synapse maintenance and full effector potential
Johannes B Huppa et al. Nat Immunol. 2003 Aug.
Abstract
Although signals through the T cell receptor (TCR) are essential for the initiation of T helper cell activation, it is unclear what function such signals have during the prolonged T cell-antigen-presenting cell contact. Here we simultaneously tracked TCR-CD3 complex and phosphoinositide 3-kinase activity in single T cells using three-dimensional video microscopy. Despite rapid internalization of most of the TCR-CD3, TCR-dependent signaling was still evident up to 10 h after conjugate formation. Blocking this interaction caused dissolution of the synapse and proportional reductions in interleukin 2 production and cellular proliferation. Thus TCR signaling persists for hours, has a cumulative effect and is necessary for the maintenance of the immunological synapse.
Comment in
- Tracking synapse-associated TCRs.
Finkel TH. Finkel TH. Nat Immunol. 2004 Feb;5(2):117; author reply 117. doi: 10.1038/ni0204-117a. Nat Immunol. 2004. PMID: 14749776 No abstract available.
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