The structure of the receptor-binding domain of the bacteriophage T4 short tail fibre reveals a knitted trimeric metal-binding fold - PubMed (original) (raw)
The structure of the receptor-binding domain of the bacteriophage T4 short tail fibre reveals a knitted trimeric metal-binding fold
Ellen Thomassen et al. J Mol Biol. 2003.
Abstract
Adsorption of T4 bacteriophage to the Escherichia coli host cell is mediated by six long and six short tail fibres. After at least three long tail fibres have bound, short tail fibres extend and bind irreversibly to the core region of the host cell lipo-polysaccharide (LPS), serving as inextensible stays during penetration of the cell envelope by the tail tube. The short tail fibres consist of a parallel, in-register, trimer of gene product 12 (gp12).X-ray crystallography at 1.5A resolution of a protease-stable fragment of gp12 generated in the presence of zinc chloride reveals the structure of the C-terminal receptor-binding domain. It has a novel "knitted" fold, consisting of three extensively intertwined monomers. It reveals a metal-binding site, containing a zinc ion coordinated by six histidine residues in an octahedral conformation. We also suggest an LPS-binding region.
Similar articles
- Crystal structure of a heat and protease-stable part of the bacteriophage T4 short tail fibre.
van Raaij MJ, Schoehn G, Burda MR, Miller S. van Raaij MJ, et al. J Mol Biol. 2001 Dec 14;314(5):1137-46. doi: 10.1006/jmbi.2000.5204. J Mol Biol. 2001. PMID: 11743729 - Identification and crystallisation of a heat- and protease-stable fragment of the bacteriophage T4 short tail fibre.
van Raaij MJ, Schoehn G, Jaquinod M, Ashman K, Burda MR, Miller S. van Raaij MJ, et al. Biol Chem. 2001 Jul;382(7):1049-55. doi: 10.1515/BC.2001.131. Biol Chem. 2001. PMID: 11530935 - Structure of bacteriophage T4 gene product 11, the interface between the baseplate and short tail fibers.
Leiman PG, Kostyuchenko VA, Shneider MM, Kurochkina LP, Mesyanzhinov VV, Rossmann MG. Leiman PG, et al. J Mol Biol. 2000 Aug 25;301(4):975-85. doi: 10.1006/jmbi.2000.3989. J Mol Biol. 2000. PMID: 10966799 - The bacteriophage T4 DNA injection machine.
Rossmann MG, Mesyanzhinov VV, Arisaka F, Leiman PG. Rossmann MG, et al. Curr Opin Struct Biol. 2004 Apr;14(2):171-80. doi: 10.1016/j.sbi.2004.02.001. Curr Opin Struct Biol. 2004. PMID: 15093831 Review. - [Bacteriophage T4: molecular aspects of bacterial cell infection and the role of capsid proteins].
Figura G, Budynek P, Dabrowska K. Figura G, et al. Postepy Hig Med Dosw (Online). 2010 May 25;64:251-61. Postepy Hig Med Dosw (Online). 2010. PMID: 20498502 Review. Polish.
Cited by
- A hybrid receptor binding protein enables phage F341 infection of Campylobacter by binding to flagella and lipooligosaccharides.
Ostenfeld LJ, Sørensen AN, Neve H, Vitt A, Klumpp J, Sørensen MCH. Ostenfeld LJ, et al. Front Microbiol. 2024 Feb 1;15:1358909. doi: 10.3389/fmicb.2024.1358909. eCollection 2024. Front Microbiol. 2024. PMID: 38380094 Free PMC article. - Diversity, Relationship, and Distribution of Virophages and Large Algal Viruses in Global Ocean Viromes.
Wu Z, Chu T, Sheng Y, Yu Y, Wang Y. Wu Z, et al. Viruses. 2023 Jul 20;15(7):1582. doi: 10.3390/v15071582. Viruses. 2023. PMID: 37515268 Free PMC article. - Grazing on Marine Viruses and Its Biogeochemical Implications.
Mayers KMJ, Kuhlisch C, Basso JTR, Saltvedt MR, Buchan A, Sandaa RA. Mayers KMJ, et al. mBio. 2023 Feb 28;14(1):e0192121. doi: 10.1128/mbio.01921-21. Epub 2023 Jan 30. mBio. 2023. PMID: 36715508 Free PMC article. Review. - Targeting of Pseudomonas aeruginosa cell surface via GP12, an Escherichia coli specific bacteriophage protein.
Ongwae GM, Chordia MD, Cawley JL, Dalesandro BE, Wittenberg NJ, Pires MM. Ongwae GM, et al. Sci Rep. 2022 Jan 14;12(1):721. doi: 10.1038/s41598-021-04627-4. Sci Rep. 2022. PMID: 35031652 Free PMC article. - Survival Comes at a Cost: A Coevolution of Phage and Its Host Leads to Phage Resistance and Antibiotic Sensitivity of Pseudomonas aeruginosa Multidrug Resistant Strains.
Koderi Valappil S, Shetty P, Deim Z, Terhes G, Urbán E, Váczi S, Patai R, Polgár T, Pertics BZ, Schneider G, Kovács T, Rákhely G. Koderi Valappil S, et al. Front Microbiol. 2021 Dec 2;12:783722. doi: 10.3389/fmicb.2021.783722. eCollection 2021. Front Microbiol. 2021. PMID: 34925289 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources