Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy - PubMed (original) (raw)
. 2003 Aug 7;424(6949):643-50.
doi: 10.1038/nature01830.
Affiliations
- PMID: 12904785
- DOI: 10.1038/nature01830
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy
Koji Yonekura et al. Nature. 2003.
Abstract
The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagellin fragment lacking about 100 terminal residues revealed the protofilament structure, but the full filament structure is still essential for understanding the mechanism of supercoiling and polymerization. Here we report a complete atomic model of the R-type filament by electron cryomicroscopy. A density map obtained from image data up to 4 A resolution shows the feature of alpha-helical backbone and some large side chains. The atomic model built on the map reveals intricate molecular packing and an alpha-helical coiled coil formed by the terminal chains in the inner core of the filament, with its intersubunit hydrophobic interactions having an important role in stabilizing the filament.
Similar articles
- Building the atomic model for the bacterial flagellar filament by electron cryomicroscopy and image analysis.
Yonekura K, Maki-Yonekura S, Namba K. Yonekura K, et al. Structure. 2005 Mar;13(3):407-12. doi: 10.1016/j.str.2005.02.003. Structure. 2005. PMID: 15766542 - Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling.
Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K. Samatey FA, et al. Nature. 2001 Mar 15;410(6826):331-7. doi: 10.1038/35066504. Nature. 2001. PMID: 11268201 - Divergence of quaternary structures among bacterial flagellar filaments.
Galkin VE, Yu X, Bielnicki J, Heuser J, Ewing CP, Guerry P, Egelman EH. Galkin VE, et al. Science. 2008 Apr 18;320(5874):382-5. doi: 10.1126/science.1155307. Science. 2008. PMID: 18420936 - [Electron cryomicroscopy of the bacterial flagellar nanomachine].
Yonekura K, Maki-Yonekura S, Namba K. Yonekura K, et al. Tanpakushitsu Kakusan Koso. 2004 Mar;49(4):534-42. Tanpakushitsu Kakusan Koso. 2004. PMID: 15024844 Review. Japanese. No abstract available. - The archaeabacterial flagellar filament: a bacterial propeller with a pilus-like structure.
Trachtenberg S, Cohen-Krausz S. Trachtenberg S, et al. J Mol Microbiol Biotechnol. 2006;11(3-5):208-20. doi: 10.1159/000094055. J Mol Microbiol Biotechnol. 2006. PMID: 16983196 Review.
Cited by
- Structural diversity and clustering of bacterial flagellar outer domains.
Fields JL, Zhang H, Bellis NF, Petersen HA, Halder SK, Rich-New ST, Krupovic M, Wu H, Wang F. Fields JL, et al. Nat Commun. 2024 Nov 3;15(1):9500. doi: 10.1038/s41467-024-53923-w. Nat Commun. 2024. PMID: 39489766 Free PMC article. - Flagella and Cell Body Staining of Bacteria with Fluorescent Dyes.
Pfeifer V, Muraveva V, Beta C. Pfeifer V, et al. Methods Mol Biol. 2024;2828:79-85. doi: 10.1007/978-1-0716-4023-4_8. Methods Mol Biol. 2024. PMID: 39147972 - Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages.
Sanchez JC, Baumgardt JK, Wright ER. Sanchez JC, et al. Bio Protoc. 2024 Jul 20;14(14):e5032. doi: 10.21769/BioProtoc.5032. eCollection 2024 Jul 20. Bio Protoc. 2024. PMID: 39100595 Free PMC article. - Systemic antibody responses against gut microbiota flagellins implicate shared and divergent immune reactivity in Crohn's disease and chronic fatigue syndrome.
Bourgonje AR, Hörstke NV, Fehringer M, Innocenti G, Vogl T. Bourgonje AR, et al. Microbiome. 2024 Jul 29;12(1):141. doi: 10.1186/s40168-024-01858-1. Microbiome. 2024. PMID: 39075559 Free PMC article. - FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria.
Halte M, Andrianova EP, Goosmann C, Chevance FFV, Hughes KT, Zhulin IB, Erhardt M. Halte M, et al. Nat Commun. 2024 Jul 14;15(1):5921. doi: 10.1038/s41467-024-50278-0. Nat Commun. 2024. PMID: 39004688 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources