The role of Ca2+ in the generation of spontaneous astrocytic Ca2+ oscillations - PubMed (original) (raw)
Comparative Study
The role of Ca2+ in the generation of spontaneous astrocytic Ca2+ oscillations
H R Parri et al. Neuroscience. 2003.
Abstract
Astrocytes in the rat thalamus display spontaneous [Ca(2+)](i) oscillations that are due to intracellular release, but are not dependent on neuronal activity. In this study we have investigated the mechanisms involved in these spontaneous [Ca(2+)](i) oscillations using slices loaded with Fluo-4 AM (5 microM) and confocal microscopy. Bafilomycin A1 incubation had no effect on the number of spontaneous [Ca(2+)](i) oscillations indicating that they were not dependent on vesicular neurotransmitter release. Oscillations were also unaffected by ryanodine. Phospholipase C (PLC) inhibition decreased the number of astrocytes responding to metabotropic glutamate receptor (mGluR) activation but did not reduce the number of spontaneously active astrocytes, indicating that [Ca(2+)](i) increases are not due to membrane-coupled PLC activation. Spontaneous [Ca(2+)](i) increases were abolished by an IP3 receptor antagonist, whilst the protein kinase C (PKC) inhibitor chelerythrine chloride prolonged their duration, indicating a role for PKC and inositol 1,4,5,-triphosphate receptor activation. BayK8644 increased the number of astrocytes exhibiting [Ca(2+)](i) oscillations, and prolonged the responses to mGluR activation, indicating a possible effect on store-operated Ca(2+) entry. Increasing [Ca(2+)](o) increased the number of spontaneously active astrocytes and the number of transients exhibited by each astrocyte. Inhibition of the endoplasmic reticulum Ca(2+) ATPase by cyclopiazonic acid also induced [Ca(2+)](i) transients in astrocytes indicating a role for cytoplasmic Ca(2+) in the induction of spontaneous oscillations. Incubation with 20 microM Fluo-4 reduced the number of astrocytes exhibiting spontaneous increases. This study indicates that Ca(2+) has a role in triggering Ca(2+) release from an inositol 1,4,5,-triphosphate sensitive store in astrocytes during the generation of spontaneous [Ca(2+)](i) oscillations.
Similar articles
- Cellular mechanism for spontaneous calcium oscillations in astrocytes.
Wang TF, Zhou C, Tang AH, Wang SQ, Chai Z. Wang TF, et al. Acta Pharmacol Sin. 2006 Jul;27(7):861-8. doi: 10.1111/j.1745-7254.2006.00397.x. Acta Pharmacol Sin. 2006. PMID: 16787570 - Regulation of store-operated calcium entry by calcium-independent phospholipase A2 in rat cerebellar astrocytes.
Singaravelu K, Lohr C, Deitmer JW. Singaravelu K, et al. J Neurosci. 2006 Sep 13;26(37):9579-92. doi: 10.1523/JNEUROSCI.2604-06.2006. J Neurosci. 2006. PMID: 16971542 Free PMC article. - Priming of long-term potentiation mediated by ryanodine receptor activation in rat hippocampal slices.
Mellentin C, Jahnsen H, Abraham WC. Mellentin C, et al. Neuropharmacology. 2007 Jan;52(1):118-25. doi: 10.1016/j.neuropharm.2006.07.009. Epub 2006 Aug 14. Neuropharmacology. 2007. PMID: 16905161 - The trinity of Ca2+ sources for the exocytotic glutamate release from astrocytes.
Reyes RC, Parpura V. Reyes RC, et al. Neurochem Int. 2009 Jul-Aug;55(1-3):2-8. doi: 10.1016/j.neuint.2008.12.018. Epub 2009 Jan 8. Neurochem Int. 2009. PMID: 19171170 Free PMC article. Review.
Cited by
- Deletion of voltage-gated calcium channels in astrocytes decreases neuroinflammation and demyelination in a murine model of multiple sclerosis.
Denaroso GE, Smith Z, Angeliu CG, Cheli VT, Wang C, Paez PM. Denaroso GE, et al. J Neuroinflammation. 2023 Nov 14;20(1):263. doi: 10.1186/s12974-023-02948-x. J Neuroinflammation. 2023. PMID: 37964385 Free PMC article. - A Dichotomous Role for FABP7 in Sleep and Alzheimer's Disease Pathogenesis: A Hypothesis.
Needham H, Torpey G, Flores CC, Davis CJ, Vanderheyden WM, Gerstner JR. Needham H, et al. Front Neurosci. 2022 Jun 30;16:798994. doi: 10.3389/fnins.2022.798994. eCollection 2022. Front Neurosci. 2022. PMID: 35844236 Free PMC article. - Neuron to Oligodendrocyte Precursor Cell Synapses: Protagonists in Oligodendrocyte Development and Myelination, and Targets for Therapeutics.
Moura DMS, Brennan EJ, Brock R, Cocas LA. Moura DMS, et al. Front Neurosci. 2022 Jan 18;15:779125. doi: 10.3389/fnins.2021.779125. eCollection 2021. Front Neurosci. 2022. PMID: 35115904 Free PMC article. Review. - Astrocytic Ca2+ Signaling in Epilepsy.
Heuser K, Enger R. Heuser K, et al. Front Cell Neurosci. 2021 Jul 15;15:695380. doi: 10.3389/fncel.2021.695380. eCollection 2021. Front Cell Neurosci. 2021. PMID: 34335188 Free PMC article. Review. - Astrocytic Calcium Dynamics Along the Pain Pathway.
Cho J, Huh Y. Cho J, et al. Front Cell Neurosci. 2020 Oct 16;14:594216. doi: 10.3389/fncel.2020.594216. eCollection 2020. Front Cell Neurosci. 2020. PMID: 33192331 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous