Single-molecule measurement of protein folding kinetics - PubMed (original) (raw)
. 2003 Aug 29;301(5637):1233-5.
doi: 10.1126/science.1085399.
Affiliations
- PMID: 12947198
- DOI: 10.1126/science.1085399
Single-molecule measurement of protein folding kinetics
Everett A Lipman et al. Science. 2003.
Abstract
In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a confocal optical system. This combination enables time-resolved measurement of Förster resonance energy transfer after an abrupt change in solution conditions. Observations of a small protein show the evolution of the intramolecular distance distribution as folding progresses. This technique can expose subpopulations, such as unfolded protein under conditions favoring the native structure, that would be obscured in equilibrium experiments.
Similar articles
- Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy.
Schuler B, Lipman EA, Eaton WA. Schuler B, et al. Nature. 2002 Oct 17;419(6908):743-7. doi: 10.1038/nature01060. Nature. 2002. PMID: 12384704 - Two-state folding observed in individual protein molecules.
Rhoades E, Cohen M, Schuler B, Haran G. Rhoades E, et al. J Am Chem Soc. 2004 Nov 17;126(45):14686-7. doi: 10.1021/ja046209k. J Am Chem Soc. 2004. PMID: 15535670 - Fast collapse but slow formation of secondary structure elements in the refolding transition of E. coli adenylate kinase.
Ratner V, Amir D, Kahana E, Haas E. Ratner V, et al. J Mol Biol. 2005 Sep 23;352(3):683-99. doi: 10.1016/j.jmb.2005.06.074. J Mol Biol. 2005. PMID: 16098987 - Coordinate and time-dependent diffusion dynamics in protein folding.
Oliveira RJ, Whitford PC, Chahine J, Leite VB, Wang J. Oliveira RJ, et al. Methods. 2010 Sep;52(1):91-8. doi: 10.1016/j.ymeth.2010.04.016. Epub 2010 May 11. Methods. 2010. PMID: 20438841 Review.
Cited by
- Two-Dimensional Fluctuation Correlation Spectroscopy (2D-FlucCS): A Method to Determine the Origin of Relaxation Rate Dispersion.
Gupta R, Verma SD. Gupta R, et al. ACS Meas Sci Au. 2024 Feb 1;4(2):153-162. doi: 10.1021/acsmeasuresciau.3c00048. eCollection 2024 Apr 17. ACS Meas Sci Au. 2024. PMID: 38645580 Free PMC article. Review. - Rapid droplet-based mixing for single-molecule spectroscopy.
Yang T, Buholzer KJ, Sottini A, Cao X, deMello A, Nettels D, Schuler B. Yang T, et al. Nat Methods. 2023 Oct;20(10):1479-1482. doi: 10.1038/s41592-023-01995-9. Epub 2023 Sep 25. Nat Methods. 2023. PMID: 37749213 - Protein folding mechanism revealed by single-molecule force spectroscopy experiments.
Sun H, Guo Z, Hong H, Yu P, Xue Z, Chen H. Sun H, et al. Biophys Rep. 2021 Oct 31;7(5):399-412. doi: 10.52601/bpr.2021.210024. Biophys Rep. 2021. PMID: 37288103 Free PMC article. - Elucidating Protein-Ligand Interactions in Cell Lysates Using High-Throughput Hydrogen-Deuterium Exchange Mass Spectrometry with Integrated Protein Thermal Depletion.
Fang M, Wu O, Cupp-Sutton KA, Smith K, Wu S. Fang M, et al. Anal Chem. 2023 Jan 6:10.1021/acs.analchem.2c04266. doi: 10.1021/acs.analchem.2c04266. Online ahead of print. Anal Chem. 2023. PMID: 36608260 Free PMC article. - The Effect of Time Resolution on Apparent Transition Path Times Observed in Single-Molecule Studies of Biomolecules.
Makarov DE, Berezhkovskii A, Haran G, Pollak E. Makarov DE, et al. J Phys Chem B. 2022 Oct 13;126(40):7966-7974. doi: 10.1021/acs.jpcb.2c05550. Epub 2022 Oct 4. J Phys Chem B. 2022. PMID: 36194758 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources