Inhibition of p38 mitogen-activated protein kinase interferes with cell shape changes and gene expression associated with Schwann cell myelination - PubMed (original) (raw)
Inhibition of p38 mitogen-activated protein kinase interferes with cell shape changes and gene expression associated with Schwann cell myelination
Gabriela Fragoso et al. Exp Neurol. 2003 Sep.
Abstract
In the present study we demonstrate that p38, a member of the mitogen-activated protein kinase (MAPK) family, is essential for ascorbate- and laminin-induced myelination in Schwann cell-dorsal root ganglion neuron cocultures. The inhibitory effect of the specific p38 blockers, PD 169316 and SB 203580, on ascorbate-induced myelination was exerted during the early stages (1-2 days) of ascorbate treatment. Inhibition of p38 was further shown to prevent the alignment of Schwann cells along axons in laminin-treated cocultures. The addition of laminin to Schwann cell-dorsal root ganglion neuron cocultures stimulated phosphorylation of p38, thereby demonstrating a link between laminin-induced myelination and p38 activation. Similarly, the small heat shock protein, Hsp27, which is phosphorylated by MAPKAPK2, a downstream substrate of p38, was phosphorylated in response to the addition of laminin to the cocultures. The p38 inhibitors did not affect the proliferation or survival of Schwann cells in the cocultures as assessed by BrdU incorporation and total cell counts. However, p38 inhibition interfered with an early stage in myelination, thereby preventing ascorbate-induced increases in the levels of mRNAs encoding MBP, MAG, and P(0) and reducing laminin deposition. These results indicate that activation of p38 by a signaling pathway(s) involving laminin and appropriate integrin receptor(s) is required for the alignment of Schwann cells with axons that precedes myelination.
Similar articles
- Mitogen-activated protein kinase p38 regulates Krox-20 to direct Schwann cell differentiation and peripheral myelination.
Hossain S, de la Cruz-Morcillo MA, Sanchez-Prieto R, Almazan G. Hossain S, et al. Glia. 2012 Jul;60(7):1130-44. doi: 10.1002/glia.22340. Epub 2012 Apr 17. Glia. 2012. PMID: 22511272 - Inhibition of Schwann cell myelination in vitro by antibody to the L1 adhesion molecule.
Wood PM, Schachner M, Bunge RP. Wood PM, et al. J Neurosci. 1990 Nov;10(11):3635-45. doi: 10.1523/JNEUROSCI.10-11-03635.1990. J Neurosci. 1990. PMID: 2230951 Free PMC article. - Lck tyrosine kinase mediates β1-integrin signalling to regulate Schwann cell migration and myelination.
Ness JK, Snyder KM, Tapinos N. Ness JK, et al. Nat Commun. 2013;4:1912. doi: 10.1038/ncomms2928. Nat Commun. 2013. PMID: 23715271 Free PMC article. - p38 Mitogen-activated protein kinase regulates myelination.
Haines JD, Fragoso G, Hossain S, Mushynski WE, Almazan G. Haines JD, et al. J Mol Neurosci. 2008 May;35(1):23-33. doi: 10.1007/s12031-007-9011-0. Epub 2007 Nov 10. J Mol Neurosci. 2008. PMID: 17994198 Review. - Regulation of Schwann cell function by the extracellular matrix.
Chernousov MA, Yu WM, Chen ZL, Carey DJ, Strickland S. Chernousov MA, et al. Glia. 2008 Nov 1;56(14):1498-1507. doi: 10.1002/glia.20740. Glia. 2008. PMID: 18803319 Review.
Cited by
- Strain-induced bands of Büngner formation promotes axon growth in 3D tissue-engineered constructs.
Hromada C, Szwarc-Hofbauer D, Quyen Nguyen M, Tomasch J, Purtscher M, Hercher D, Teuschl-Woller AH. Hromada C, et al. J Tissue Eng. 2024 Jan 18;15:20417314231220396. doi: 10.1177/20417314231220396. eCollection 2024 Jan-Dec. J Tissue Eng. 2024. PMID: 38249993 Free PMC article. - The proteome of distal nerves: implication in delayed repair and poor functional recovery.
Guo S, Moore RM, Charlesworth MC, Johnson KL, Spinner RJ, Windebank AJ, Wang H. Guo S, et al. Neural Regen Res. 2022 Sep;17(9):1998-2006. doi: 10.4103/1673-5374.335159. Neural Regen Res. 2022. PMID: 35142689 Free PMC article. - Expression and Function of GABA Receptors in Myelinating Cells.
Serrano-Regal MP, Bayón-Cordero L, Ordaz RP, Garay E, Limon A, Arellano RO, Matute C, Sánchez-Gómez MV. Serrano-Regal MP, et al. Front Cell Neurosci. 2020 Aug 21;14:256. doi: 10.3389/fncel.2020.00256. eCollection 2020. Front Cell Neurosci. 2020. PMID: 32973453 Free PMC article. - The p38α MAPK Deletion in Oligodendroglia does not Attenuate Myelination Defects in a Mouse Model of Periventricular Leukomalacia.
Chung SH, Biswas S, Sohn J, Jiang P, Dehghan S, Marzban H, Deng W. Chung SH, et al. Neuroscience. 2018 Aug 21;386:175-181. doi: 10.1016/j.neuroscience.2018.06.037. Epub 2018 Jul 5. Neuroscience. 2018. PMID: 29966722 Free PMC article. - Tissue Inhibitor of Metalloproteinase-3 Promotes Schwann Cell Myelination.
Kim J, Elias A, Lee T, Maurel P, Kim HA. Kim J, et al. ASN Neuro. 2017 Nov-Dec;9(6):1759091417745425. doi: 10.1177/1759091417745425. ASN Neuro. 2017. PMID: 29198135 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous