Involvement of a cGMP-dependent pathway in the natriuretic peptide-mediated hormone-sensitive lipase phosphorylation in human adipocytes - PubMed (original) (raw)
. 2003 Dec 5;278(49):48617-26.
doi: 10.1074/jbc.M303713200. Epub 2003 Sep 10.
Affiliations
- PMID: 12970365
- DOI: 10.1074/jbc.M303713200
Free article
Involvement of a cGMP-dependent pathway in the natriuretic peptide-mediated hormone-sensitive lipase phosphorylation in human adipocytes
Coralie Sengenes et al. J Biol Chem. 2003.
Free article
Abstract
Our previous studies have demonstrated that natriuretic peptides (NPs), peptide hormones with natriuretic, diuretic, and vasodilating properties, exert a potent control on the lipolysis in human adipocytes via the activation of the type A guanylyl cyclase receptor (1, 2). In the current study we investigated the intracellular mechanisms involved in the NP-stimulated lipolytic effect in human preadipocytes and adipocytes. We demonstrate that the atrial NP (ANP)-induced lipolysis in human adipocytes was associated with an enhanced serine phosphorylation of the hormone-sensitive lipase (HSL). Both ANP-mediated lipolysis and HSL phosphorylation were inhibited in the presence of increasing concentrations of the guanylyl cyclase inhibitor LY-83583. ANP did not modulate the activity of the cAMP-dependent protein kinase (PKA). Moreover, H-89, a PKA inhibitor, did not affect the ANP-induced lipolysis. On primary cultures of human preadipocytes, the ANP-mediated lipolytic effect was dependent on the differentiation process. On differentiated human preadipocytes, ANP-mediated lipolysis, associated with an increased phosphorylation of HSL and of perilipin A, was strongly decreased by treatment with the inhibitor of the cGMP-dependent protein kinase I (cGKI), Rp-8-pCPT-cGMPS. Thus, ANP-induced lipolysis in human adipocytes is a cGMP-dependent pathway that induces the phosphorylation of HSL and perilipin A via the activation of cGKI. The present study shows that lipolysis in human adipocytes can be controlled by an independent cGKI-mediated signaling as well as by the classical cAMP/PKA pathway.
Similar articles
- Parathyroid hormone induces adipocyte lipolysis via PKA-mediated phosphorylation of hormone-sensitive lipase.
Larsson S, Jones HA, Göransson O, Degerman E, Holm C. Larsson S, et al. Cell Signal. 2016 Mar;28(3):204-213. doi: 10.1016/j.cellsig.2015.12.012. Epub 2015 Dec 23. Cell Signal. 2016. PMID: 26724218 - [Natriuretic peptides: a new lipolytic pathway in human fat cells].
Sengenes C, Moro C, Galitzky J, Berlan M, Lafontan M. Sengenes C, et al. Med Sci (Paris). 2005 Dec;21 Spec No:29-33. Med Sci (Paris). 2005. PMID: 16598902 Review. French. - Perilipin promotes hormone-sensitive lipase-mediated adipocyte lipolysis via phosphorylation-dependent and -independent mechanisms.
Miyoshi H, Souza SC, Zhang HH, Strissel KJ, Christoffolete MA, Kovsan J, Rudich A, Kraemer FB, Bianco AC, Obin MS, Greenberg AS. Miyoshi H, et al. J Biol Chem. 2006 Jun 9;281(23):15837-44. doi: 10.1074/jbc.M601097200. Epub 2006 Apr 4. J Biol Chem. 2006. PMID: 16595669 - Extract of Paecilomyces hepiali mycelia induces lipolysis through PKA-mediated phosphorylation of hormone-sensitive lipase and ERK-mediated downregulation of perilipin in 3T3-L1 adipocytes.
Ge M, Guo R, Lou HX, Zhang W. Ge M, et al. BMC Complement Altern Med. 2018 Dec 7;18(1):326. doi: 10.1186/s12906-018-2389-0. BMC Complement Altern Med. 2018. PMID: 30526586 Free PMC article. - [Natriuretic peptides: a new lipolytic pathway in human fat cells].
Sengenes C, Moro C, Galitzky J, Berlan M, Lafontan M. Sengenes C, et al. Med Sci (Paris). 2005 Jan;21(1):61-5. doi: 10.1051/medsci/200521161. Med Sci (Paris). 2005. PMID: 15639022 Review. French.
Cited by
- Activation of natriuretic peptides and the sympathetic nervous system following Roux-en-Y gastric bypass is associated with gonadal adipose tissues browning.
Neinast MD, Frank AP, Zechner JF, Li Q, Vishvanath L, Palmer BF, Aguirre V, Gupta RK, Clegg DJ. Neinast MD, et al. Mol Metab. 2015 Mar 3;4(5):427-36. doi: 10.1016/j.molmet.2015.02.006. eCollection 2015 May. Mol Metab. 2015. PMID: 25973390 Free PMC article. - Guanylin and uroguanylin stimulate lipolysis in human visceral adipocytes.
Rodríguez A, Gómez-Ambrosi J, Catalán V, Ezquerro S, Méndez-Giménez L, Becerril S, Ibáñez P, Vila N, Margall MA, Moncada R, Valentí V, Silva C, Salvador J, Frühbeck G. Rodríguez A, et al. Int J Obes (Lond). 2016 Sep;40(9):1405-15. doi: 10.1038/ijo.2016.66. Epub 2016 Apr 25. Int J Obes (Lond). 2016. PMID: 27108812 - Update on the synergistic effect of HSL and insulin in the treatment of metabolic disorders.
Lan YL, Lou JC, Lyu W, Zhang B. Lan YL, et al. Ther Adv Endocrinol Metab. 2019 Sep 20;10:2042018819877300. doi: 10.1177/2042018819877300. eCollection 2019. Ther Adv Endocrinol Metab. 2019. PMID: 31565213 Free PMC article. Review. - GREBP, a cGMP-response element-binding protein repressing the transcription of natriuretic peptide receptor 1 (NPR1/GCA).
Martel G, Hamet P, Tremblay J. Martel G, et al. J Biol Chem. 2010 Jul 2;285(27):20926-39. doi: 10.1074/jbc.M109.061622. Epub 2010 May 5. J Biol Chem. 2010. PMID: 20444705 Free PMC article. - Naringenin Increases Insulin Sensitivity and Metabolic Rate: A Case Study.
Murugesan N, Woodard K, Ramaraju R, Greenway FL, Coulter AA, Rebello CJ. Murugesan N, et al. J Med Food. 2020 Mar;23(3):343-348. doi: 10.1089/jmf.2019.0216. Epub 2019 Oct 31. J Med Food. 2020. PMID: 31670603 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous