Regional and temporal alterations in Ca2+/calmodulin-dependent protein kinase II and calcineurin in the hippocampus of rat brain after transient forebrain ischemia - PubMed (original) (raw)

Regional and temporal alterations in Ca2+/calmodulin-dependent protein kinase II and calcineurin in the hippocampus of rat brain after transient forebrain ischemia

M Morioka et al. J Neurochem. 1992 May.

Abstract

We have investigated regional and temporal alterations in Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) and calcineurin (Ca2+/calmodulin-dependent protein phosphatase) after transient forebrain ischemia. Immunoreactivity and enzyme activity of CaM kinase II decreased in regions CA1 and CA3, and in the dentate gyrus, of the hippocampus early (6-12 h) after ischemia, but the decrease in immunoreactivity gradually recovered over time, except in the CA1 region. Furthermore, the increase in Ca2+/calmodulin-independent activity was detected up to 3 days after ischemia in all regions tested, suggesting that the concentration of intracellular Ca2+ increased. In contrast to CaM kinase II, as immunohistochemistry and regional immunoblot analysis revealed, calcineurin was preserved in the CA1 region until 1.5 days and then lost with the increase in morphological degeneration of neurons. Immunoblot analysis confirmed the findings of the immunohistochemistry. These results suggest that there is a difference between CaM kinase II and calcineurin in regional and temporal loss after ischemia and that imbalance of Ca2+/calmodulin-dependent protein phosphorylation-dephosphorylation may occur.

PubMed Disclaimer

Cited by

Alpha-CaMKII deficiency causes immature dentate gyrus, a novel candidate endophenotype of psychiatric disorders.
Yamasaki N, Maekawa M, Kobayashi K, Kajii Y, Maeda J, Soma M, Takao K, Tanda K, Ohira K, Toyama K, Kanzaki K, Fukunaga K, Sudo Y, Ichinose H, Ikeda M, Iwata N, Ozaki N, Suzuki H, Higuchi M, Suhara T, Yuasa S, Miyakawa T. Yamasaki N, et al. Mol Brain. 2008 Sep 10;1:6. doi: 10.1186/1756-6606-1-6. Mol Brain. 2008. PMID: 18803808 Free PMC article.
Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction.
Lu Y, Chen X, Liu X, Shi Y, Wei Z, Feng L, Jiang Q, Ye W, Sasaki T, Fukunaga K, Ji Y, Han F, Lu YM. Lu Y, et al. Autophagy. 2023 Jun;19(6):1803-1820. doi: 10.1080/15548627.2022.2162244. Epub 2023 Jan 1. Autophagy. 2023. PMID: 36588318 Free PMC article.
Alterations of CaMKII after hypoxia-ischemia during brain development.
Tang K, Liu C, Kuluz J, Hu B. Tang K, et al. J Neurochem. 2004 Oct;91(2):429-37. doi: 10.1111/j.1471-4159.2004.02733.x. J Neurochem. 2004. PMID: 15447676 Free PMC article.
Ouabain induces apoptotic cell death in type I spiral ganglion neurons, but not type II neurons.
Lang H, Schulte BA, Schmiedt RA. Lang H, et al. J Assoc Res Otolaryngol. 2005 Mar;6(1):63-74. doi: 10.1007/s10162-004-5021-6. Epub 2005 Apr 22. J Assoc Res Otolaryngol. 2005. PMID: 15735933 Free PMC article.
Remote Ischemia Postconditioning Mitigates Hippocampal Neuron Impairment by Modulating Cav1.2-CaMKIIα-Aromatase Signaling After Global Cerebral Ischemia in Ovariectomized Rats.
Wang L, Gao F, Chen L, Sun W, Liu H, Yang W, Zhang X, Bai J, Wang R. Wang L, et al. Mol Neurobiol. 2024 Sep;61(9):6511-6527. doi: 10.1007/s12035-024-03930-1. Epub 2024 Feb 6. Mol Neurobiol. 2024. PMID: 38321351 Free PMC article.

Regional and temporal alterations in Ca2+/calmodulin-dependent protein kinase II and calcineurin in the hippocampus of rat brain after transient forebrain ischemia - PubMed (original) (raw)