enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells - PubMed (original) (raw)

Affiliations

PMID: 1323817

smg/rap1/Krev-1 p21s inhibit the signal pathway to the c-fos promoter/enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells

T Sakoda et al. Oncogene. 1992 Sep.

Abstract

smg/rap1A/Krev-1 p21 cDNA is known to inhibit v-Ki-ras p21-induced cell transformation in NIH3T3 cells, but the inhibitory mechanism is not clear at present. In the present study, we examined the effect of smg p21s on the c-fos promoter/enhancer linked to the luciferase reporter gene (c-fos-luciferase). After transfection of c-fos-luciferase into NIH3T3 cells constitutively expressing c-Ki-ras(val-12) p21 or activated c-raf-1 kinase, expression of c-fos-luciferase was much higher than after transfection into control NIH3T3 cells. Addition of platelet-derived growth factor (PDGF), 12-O-tetradecanoyl phorbol 13-acetate (TPA) or dibutyryl cyclic AMP (Bt2cAMP) to the control NIH3T3 cells stimulated c-fos-luciferase expression. Transfection of the smg p21 cDNAs inhibited the activated ras p21-, PDGF- or TPA-stimulated c-fos-luciferase expression, but did not inhibit the activated c-raf-1 kinase- or Bt2cAMP-stimulated reaction. These results indicate that smg p21s inhibit the signal pathways from the PDGF receptor, protein kinase C, and ras p21s to the c-fos promoter/enhancer, but not those from c-raf-1 kinase and cyclic AMP-dependent protein kinase to the c-fos promoter/enhancer.

PubMed Disclaimer

Cited by

Rap1 and its effector KRIT1/CCM1 regulate beta-catenin signaling.
Glading AJ, Ginsberg MH. Glading AJ, et al. Dis Model Mech. 2010 Jan-Feb;3(1-2):73-83. doi: 10.1242/dmm.003293. Epub 2009 Dec 9. Dis Model Mech. 2010. PMID: 20007487 Free PMC article.
Rig is a novel Ras-related protein and potential neural tumor suppressor.
Ellis CA, Vos MD, Howell H, Vallecorsa T, Fults DW, Clark GJ. Ellis CA, et al. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9876-81. doi: 10.1073/pnas.142193799. Epub 2002 Jul 9. Proc Natl Acad Sci U S A. 2002. PMID: 12107278 Free PMC article.
Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade.
Ohba Y, Mochizuki N, Matsuo K, Yamashita S, Nakaya M, Hashimoto Y, Hamaguchi M, Kurata T, Nagashima K, Matsuda M. Ohba Y, et al. Mol Cell Biol. 2000 Aug;20(16):6074-83. doi: 10.1128/MCB.20.16.6074-6083.2000. Mol Cell Biol. 2000. PMID: 10913189 Free PMC article.
Extracellular signal-regulated activation of Rap1 fails to interfere in Ras effector signalling.
Zwartkruis FJ, Wolthuis RM, Nabben NM, Franke B, Bos JL. Zwartkruis FJ, et al. EMBO J. 1998 Oct 15;17(20):5905-12. doi: 10.1093/emboj/17.20.5905. EMBO J. 1998. PMID: 9774335 Free PMC article.
Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis.
Ohba Y, Ikuta K, Ogura A, Matsuda J, Mochizuki N, Nagashima K, Kurokawa K, Mayer BJ, Maki K, Miyazaki J, Matsuda M. Ohba Y, et al. EMBO J. 2001 Jul 2;20(13):3333-41. doi: 10.1093/emboj/20.13.3333. EMBO J. 2001. PMID: 11432821 Free PMC article.

smg/rap1/Krev-1 p21s inhibit the signal pathway to the c-fos promoter/enhancer from c-Ki-ras p21 but not from c-raf-1 kinase in NIH3T3 cells - PubMed (original) (raw)