New thermosensitive plasmid for gram-positive bacteria - PubMed (original) (raw)

Comparative Study

New thermosensitive plasmid for gram-positive bacteria

E Maguin et al. J Bacteriol. 1992 Sep.

Abstract

We isolated a replication-thermosensitive mutant of the broad-host-range replicon pWV01. The mutant pVE6002 is fully thermosensitive above 35 degrees C in both gram-negative and gram-positive bacteria. Four clustered mutations were identified in the gene encoding the replication protein of pVE6002. The thermosensitive derivative of the related plasmid pE194 carries a mutation in the analogous region but not in the same position. Derivatives of the thermosensitive plasmid convenient for cloning purposes have been constructed. The low shut-off temperature of pVE6002 makes it a useful suicide vector for bacteria which are limited in their own temperature growth range. Using pVE6002 as the delivery vector for a transposon Tn10 derivative in Bacillus subtilis, we observed transposition frequencies of about 1%.

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References

    1. EMBO J. 1992 Apr;11(4):1317-26 - PubMed
    1. Appl Environ Microbiol. 1992 Jan;58(1):187-93 - PubMed
    1. Mol Gen Genet. 1991 May;227(1):33-9 - PubMed
    1. Nucleic Acids Res. 1990 Aug 25;18(16):4651-7 - PubMed
    1. J Bacteriol. 1990 Dec;172(12):6736-40 - PubMed

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